De Wael K, Van Dijck K, Gason F
Nationaal Instituut voor Criminalistiek en Criminologie, Vilvoordsesteenweg 100, B-1120 Brussel, Belgium.
Nationaal Instituut voor Criminalistiek en Criminologie, Vilvoordsesteenweg 100, B-1120 Brussel, Belgium. Electronic address: http://www.nicc.fgov.be.
Sci Justice. 2015 Dec;55(6):422-30. doi: 10.1016/j.scijus.2015.06.001. Epub 2015 Jun 26.
Reactively-dyed black, navy blue and medium red cotton samples showing metamerism under fluorescent tube illumination were examined. Optical microscopy (bright field, polarization and fluorescence microscopy) was used, followed by microspectrometry in the visible range (MSP Vis), to differentiate the samples in each block of colours. Additionally, the non-discriminated samples were subjected both to microspectrophotometry in the UV-range (MSP UV) and to enzymatic digestion followed by high performance thin layer chromatography (HPTLC) on the digests. While it was found that both methods may potentially result in higher discrimination, preparation of reactively-dyed cotton for HPTLC was found to be a very tedious and time-consuming step and HPTLC only led to a better discrimination than MSP UV for the red cotton samples. The results suggest that in order to increase the discrimination for reactively-dyed cotton fibres, measurement of the UV absorption spectrum (MSP UV) is preferred over HPTLC.
对在荧光灯管照射下呈现同色异谱现象的活性染色黑色、藏青色和中红色棉样品进行了检测。使用了光学显微镜（明场、偏振和荧光显微镜），随后进行可见光谱范围内的显微光谱分析（MSP Vis），以区分每种颜色组中的样品。此外，对未区分开的样品进行了紫外光谱范围内的显微分光光度测定（MSP UV），并对酶解产物进行了高效薄层色谱分析（HPTLC）。虽然发现这两种方法都可能带来更高的区分度，但发现活性染色棉用于HPTLC的制备是一个非常繁琐且耗时的步骤，并且HPTLC仅对红色棉样品的区分效果优于MSP UV。结果表明，为了提高对活性染色棉纤维的区分度，紫外吸收光谱测量（MSP UV）比HPTLC更可取。
