Was-Gubala Jolanta, Starczak Roza
Institute of Forensic Research, Department of Criminalistics, Westerplatte 9, 31-033 Krakow, Poland.
Institute of Forensic Research, Department of Criminalistics, Westerplatte 9, 31-033 Krakow, Poland; Jagiellonian University, Department of Analytical Chemistry, Ingardena 3, 30-060 Krakow, Poland.
Spectrochim Acta A Mol Biomol Spectrosc. 2015 May 5;142:118-25. doi: 10.1016/j.saa.2015.01.116. Epub 2015 Feb 9.
The main purposes of this study was to assess the usefulness of microspectrophotometry (MSP), both in the ultraviolet (UV) and visible (Vis) range for discriminating single cotton fibres dyed with reactive dyes coming from the same manufacturer, as well as the possibility of evaluation of the concentration of dye in an examine fibre. This study utilised woven cotton fabrics dyed with different concentrations of one-compound reactive dyes with the commercial name Cibacron® (at present Novacron®) as the focus of the MSP analysis. The spectra were recorded in the UV-Vis range between 200 and 800nm, in transmission mode. The results from this study illustrated that all of the analysed cotton samples dyed with reactive dyes were distinguishable between each other with the use of MSP, mostly in the visible, and also in ultraviolet range. The limit for applied MSP techniques was 0.18% of the concentration of a dye in the textile sample. The results indicate that based on the absorbance measurements for fibres constituting e.g. forensic traces it was not possible to estimate the concentration of the dye in the fibre because Beer's law did not obey. The intra-sample, and inter- sample variation, as well as dichroism effect in a case of a cotton fibres dyed with reactive dye were observed. On the basis of the results obtained for each analysed cotton sample, it was concluded that there was no correlation between colour uniformity in cotton fabric (changes in lightness, red/green and yellow/blue colour) and concentration of the reactive dye.
本研究的主要目的是评估显微分光光度法（MSP）在紫外（UV）和可见光（Vis）范围内鉴别来自同一制造商的活性染料染色的单根棉纤维的有用性，以及评估被检测纤维中染料浓度的可能性。本研究以用商品名为汽巴克隆（Cibacron®，目前为诺瓦克隆（Novacron®））的单一组分活性染料不同浓度染色的机织棉织物作为MSP分析的重点。光谱在200至800nm的紫外-可见范围内以透射模式记录。本研究结果表明，使用MSP可以区分所有用活性染料染色的分析棉样品，大多是在可见光范围内以及紫外范围内。所应用的MSP技术的限度是纺织品样品中染料浓度为0.18%。结果表明，基于对构成例如法医痕迹的纤维的吸光度测量，无法估计纤维中染料的浓度，因为不符合比尔定律。观察到了样品内和样品间的变化，以及活性染料染色棉纤维情况下的二向色性效应。根据对每个分析棉样品获得的结果得出结论，棉织物的颜色均匀性（明度、红/绿和黄/蓝颜色变化）与活性染料浓度之间没有相关性。
