Zuppinger Christian
Cardiology, Bern University Hospital, Department of Clinical Research, MEM G803b, Murtenstrasse 35, CH-3008, Bern, Switzerland.
Biochim Biophys Acta. 2016 Jul;1863(7 Pt B):1873-81. doi: 10.1016/j.bbamcr.2015.11.036. Epub 2015 Dec 2.
This review discusses historical milestones, recent developments and challenges in the area of 3D culture models with cardiovascular cell types. Expectations in this area have been raised in recent years, but more relevant in vitro research, more accurate drug testing results, reliable disease models and insights leading to bioartificial organs are expected from the transition to 3D cell culture. However, the construction of organ-like cardiac 3D models currently remains a difficult challenge. The heart consists of highly differentiated cells in an intricate arrangement.Furthermore, electrical “wiring”, a vascular system and multiple cell types act in concert to respond to the rapidly changing demands of the body. Although cardiovascular 3D culture models have been predominantly developed for regenerative medicine in the past, their use in drug screening and for disease models has become more popular recently. Many sophisticated 3D culture models are currently being developed in this dynamic area of life science. This article is part of a Special Issue entitled: Cardiomyocyte Biology: Integration of Developmental and Environmental Cues in the Heart edited by Marcus Schaub and Hughes Abriel.
本综述讨论了包含心血管细胞类型的3D培养模型领域的历史里程碑、近期进展和挑战。近年来，该领域的期望有所提高，但从向3D细胞培养的转变中，人们期望获得更相关的体外研究、更准确的药物测试结果、可靠的疾病模型以及通向生物人工器官的见解。然而，构建类器官心脏3D模型目前仍然是一项艰巨的挑战。心脏由高度分化的细胞以复杂的排列方式组成。此外，电“线路”、血管系统和多种细胞类型协同作用，以响应身体快速变化的需求。尽管心血管3D培养模型过去主要是为再生医学而开发的，但它们在药物筛选和疾病模型中的应用最近变得更加普遍。在这个充满活力的生命科学领域，目前正在开发许多复杂的3D培养模型。本文是名为《心肌细胞生物学：心脏发育和环境信号的整合》特刊的一部分，由马库斯·绍布和休斯·阿布里尔编辑。
