Gómez-Lagunas F, Peña A, Liévano A, Darszon A
Instituto de Fisiología Celular, Universidad Nacional Autónoma de México.
Biophys J. 1989 Jul;56(1):115-9. doi: 10.1016/S0006-3495(89)82656-6.
Recently, patch-clamping of yeast protoplasts has revealed the presence of plasma membrane K+ channels (Gustin, M. C., B. Martinac, Y. Saimi, M. R. Culberston, and C. Kung. 1986. Science (Wash. DC). 233:1195-1197). In this work we show that fusion of purified plasma membranes into planar bilayers allows the study of the yeast channels. The main cationic conductances detected were of 64 and 116 pS, however, larger and smaller conductances have been observed. The two main conductances were sensitive to the K+ channels blockers tetraethylammonium (TEA+) and Ba2+. Bionic experiments indicated that both conductances were K+ selective.
最近，对酵母原生质体进行膜片钳实验揭示了质膜钾离子通道的存在（古斯汀，M.C.，B. 马尔蒂纳克，Y. 赛米，M.R. 卡尔伯斯顿，以及C. 孔。1986年。《科学》（华盛顿特区）。233:1195 - 1197）。在这项研究中，我们表明将纯化的质膜融合到平面双分子层中能够对酵母通道进行研究。检测到的主要阳离子电导分别为64和116皮西门子，不过，也观察到了更大和更小的电导。这两种主要电导对钾离子通道阻滞剂四乙铵（TEA +）和钡离子敏感。生物物理学实验表明这两种电导都具有钾离子选择性。
