Franzusoff A J, Cirillo V P
J Biol Chem. 1983 Mar 25;258(6):3608-14.
Purified plasma membranes prepared from yeast cells by mechanical agitation with glass beads exhibit no detectable sugar transport activity. However, the addition of phospholipid (asolectin) liposomes to the purified plasma membranes followed by freezing, thawing, and brief sonication produces membrane vesicles which exhibit D-glucose-specific transport activity. The characteristics of zero trans, equilibrium exchange, and influx counterflow exhibited by the membrane vesicles are similar to those of intact cells.
通过与玻璃珠机械搅拌从酵母细胞制备的纯化质膜未表现出可检测到的糖转运活性。然而，向纯化的质膜中加入磷脂（大豆卵磷脂）脂质体，然后进行冷冻、解冻和短暂超声处理，会产生具有D-葡萄糖特异性转运活性的膜泡。膜泡表现出的零转运、平衡交换和流入逆流特性与完整细胞的特性相似。
