用于检测囊性纤维化患者中铜绿假单胞菌草原流行株的PCR检测方法的开发与验证
Development and Validation of a PCR Assay To Detect the Prairie Epidemic Strain of Pseudomonas aeruginosa from Patients with Cystic Fibrosis.
作者信息
Workentine M, Poonja A, Waddell B, Duong J, Storey D G, Gregson D, Somayaji R, Rabin H R, Surette M G, Parkins M D
机构信息
Department of Veterinary Medicine, University of Calgary, Calgary, Canada.
Department of Microbiology, Immunology, and Infectious Diseases, University of Calgary, Calgary, Canada.
出版信息
J Clin Microbiol. 2016 Feb;54(2):489-91. doi: 10.1128/JCM.02603-15. Epub 2015 Dec 9.
Abstract
The monitoring of epidemic Pseudomonas aeruginosa is important for cystic fibrosis (CF) infection control. The prairie epidemic strain (PES) is common in western Canadian CF clinics. Using whole-genome sequencing, we identified a novel genomic island and developed a PCR assay for PES. Against a collection of 186 P. aeruginosa isolates, the assay had 98% sensitivity and 100% specificity.
摘要
监测流行的铜绿假单胞菌对于囊性纤维化（CF）感染控制很重要。草原流行菌株（PES）在加拿大西部的CF诊所中很常见。通过全基因组测序，我们鉴定出一个新的基因组岛，并开发了一种针对PES的PCR检测方法。针对186株铜绿假单胞菌分离株的集合，该检测方法的灵敏度为98%，特异性为100%。
相似文献
1
Development and Validation of a PCR Assay To Detect the Prairie Epidemic Strain of Pseudomonas aeruginosa from Patients with Cystic Fibrosis.
J Clin Microbiol. 2016 Feb;54(2):489-91. doi: 10.1128/JCM.02603-15. Epub 2015 Dec 9.
2
Twenty-five-year outbreak of Pseudomonas aeruginosa infecting individuals with cystic fibrosis: identification of the prairie epidemic strain.
J Clin Microbiol. 2014 Apr;52(4):1127-35. doi: 10.1128/JCM.03218-13. Epub 2014 Jan 22.
3
Comparison of three typing methods for Pseudomonas aeruginosa isolates from patients with cystic fibrosis.
Eur J Clin Microbiol Infect Dis. 2012 Dec;31(12):3341-50. doi: 10.1007/s10096-012-1701-z. Epub 2012 Jul 29.
4
Long-term clinical outcomes of 'Prairie Epidemic Strain' infection in adults with cystic fibrosis.
Thorax. 2017 Apr;72(4):333-339. doi: 10.1136/thoraxjnl-2015-208083. Epub 2016 Sep 28.
5
Comparison of three molecular techniques for typing Pseudomonas aeruginosa isolates in sputum samples from patients with cystic fibrosis.
J Clin Microbiol. 2011 Jan;49(1):263-8. doi: 10.1128/JCM.01421-10. Epub 2010 Nov 17.
6
Impact of Pseudomonas aeruginosa genomic instability on the application of typing methods for chronic cystic fibrosis infections.
J Clin Microbiol. 2010 Jun;48(6):2053-9. doi: 10.1128/JCM.00019-10. Epub 2010 Apr 21.
7
Random amplified polymorphic DNA typing of Pseudomonas aeruginosa isolates recovered from patients with cystic fibrosis.
J Clin Microbiol. 1996 May;34(5):1129-35. doi: 10.1128/jcm.34.5.1129-1135.1996.
8
Comparison of arbitrarily primed PCR and macrorestriction (pulsed-field gel electrophoresis) typing of Pseudomonas aeruginosa strains from cystic fibrosis patients.
J Clin Microbiol. 1995 Aug;33(8):2216-9. doi: 10.1128/jcm.33.8.2216-2219.1995.
9
Development of a diagnostic test for the Midlands 1 cystic fibrosis epidemic strain of Pseudomonas aeruginosa.
J Med Microbiol. 2006 Aug;55(Pt 8):1085-1091. doi: 10.1099/jmm.0.46604-0.
10
A comparison of two informative SNP-based strategies for typing Pseudomonas aeruginosa isolates from patients with cystic fibrosis.
BMC Infect Dis. 2014 Jun 5;14:307. doi: 10.1186/1471-2334-14-307.
引用本文的文献
1
Cystic Fibrosis Patients Infected With Epidemic Strains Have Unique Microbial Communities.
Front Cell Infect Microbiol. 2020 Apr 24;10:173. doi: 10.3389/fcimb.2020.00173. eCollection 2020.
2
Epidemiology, Biology, and Impact of Clonal Pseudomonas aeruginosa Infections in Cystic Fibrosis.
Clin Microbiol Rev. 2018 Aug 29;31(4). doi: 10.1128/CMR.00019-18. Print 2018 Oct.
3
Phenotypic and Genotypic Comparison of Epidemic and Non-Epidemic Strains of Pseudomonas aeruginosa from Individuals with Cystic Fibrosis.
PLoS One. 2015 Nov 23;10(11):e0143466. doi: 10.1371/journal.pone.0143466. eCollection 2015.
4
Infection control knowledge, beliefs and behaviours amongst cystic fibrosis patients with epidemic Pseudomonas aeruginosa.
BMC Pulm Med. 2015 Nov 5;15:138. doi: 10.1186/s12890-015-0116-x.
本文引用的文献
1
Long-term clinical outcomes of 'Prairie Epidemic Strain' infection in adults with cystic fibrosis.
Thorax. 2017 Apr;72(4):333-339. doi: 10.1136/thoraxjnl-2015-208083. Epub 2016 Sep 28.
2
Successful implementation of infection control strategies prevents P. aeruginosa transmission among cystic fibrosis patients inside the hospital.
GMS Hyg Infect Control. 2014 Sep 30;9(3):Doc20. doi: 10.3205/dgkh000240. eCollection 2014.
3
Infection prevention and control guideline for cystic fibrosis: 2013 update.
Infect Control Hosp Epidemiol. 2014 Aug;35 Suppl 1:S1-S67. doi: 10.1086/676882. Epub 2014 Jul 1.
4
Twenty-five-year outbreak of Pseudomonas aeruginosa infecting individuals with cystic fibrosis: identification of the prairie epidemic strain.
J Clin Microbiol. 2014 Apr;52(4):1127-35. doi: 10.1128/JCM.03218-13. Epub 2014 Jan 22.
5
Assembling single-cell genomes and mini-metagenomes from chimeric MDA products.
J Comput Biol. 2013 Oct;20(10):714-37. doi: 10.1089/cmb.2013.0084.
6
IslandViewer update: Improved genomic island discovery and visualization.
Nucleic Acids Res. 2013 Jul;41(Web Server issue):W129-32. doi: 10.1093/nar/gkt394. Epub 2013 May 15.
7
Halting the spread of epidemic pseudomonas aeruginosa in an adult cystic fibrosis centre: a prospective cohort study.
JRSM Short Rep. 2013 Jan;4(1):1. doi: 10.1258/shorts.2012.012018. Epub 2013 Jan 14.
8
Comparison of three typing methods for Pseudomonas aeruginosa isolates from patients with cystic fibrosis.
Eur J Clin Microbiol Infect Dis. 2012 Dec;31(12):3341-50. doi: 10.1007/s10096-012-1701-z. Epub 2012 Jul 29.
9
Primer3--new capabilities and interfaces.
Nucleic Acids Res. 2012 Aug;40(15):e115. doi: 10.1093/nar/gks596. Epub 2012 Jun 22.
10
Comparison of three molecular techniques for typing Pseudomonas aeruginosa isolates in sputum samples from patients with cystic fibrosis.
J Clin Microbiol. 2011 Jan;49(1):263-8. doi: 10.1128/JCM.01421-10. Epub 2010 Nov 17.
Zotero 插件