囊性纤维化患者来源的铜绿假单胞菌菌株的任意引物PCR分型与酶切大片段DNA分型（脉冲场凝胶电泳）比较
Comparison of arbitrarily primed PCR and macrorestriction (pulsed-field gel electrophoresis) typing of Pseudomonas aeruginosa strains from cystic fibrosis patients.
作者信息
Kersulyte D, Struelens M J, Deplano A, Berg D E
机构信息
Department of Molecular Microbiology, Washington University Medical School, St. Louis, Missouri 63110, USA.
出版信息
J Clin Microbiol. 1995 Aug;33(8):2216-9. doi: 10.1128/jcm.33.8.2216-2219.1995.
Abstract
Arbitrarily primed PCR fingerprinting was carried out on 43 Pseudomonas aeruginosa isolates from cystic fibrosis (CF) patients. Seventeen major groups of strains that coincided with groups also distinguished by macrorestriction (pulsed-field gel electrophoresis) typing were identified. Our results illustrated that a CF patient can carry more than one strain and can carry a given strain for long periods of time and that strains can evolve by changes in drug resistance or other phenotypic traits during long-term colonization. The arbitrarily primed PCR method is recommended for first-pass screening of P. aeruginosa isolates from CF patients, especially when many strains are to be typed, because of its sensitivity and efficiency.
摘要
对来自囊性纤维化（CF）患者的43株铜绿假单胞菌分离株进行了任意引物PCR指纹分析。鉴定出了17个主要菌株组，这些组与通过宏观限制性酶切（脉冲场凝胶电泳）分型所区分的组一致。我们的结果表明，CF患者可能携带不止一种菌株，并且可以长时间携带某一特定菌株，而且菌株在长期定植过程中可能会因耐药性或其他表型特征的变化而发生进化。由于任意引物PCR方法具有灵敏度和高效性，因此推荐用于对CF患者的铜绿假单胞菌分离株进行初步筛选，尤其是在需要对许多菌株进行分型时。
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