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Comparison of arbitrarily primed PCR and macrorestriction (pulsed-field gel electrophoresis) typing of Pseudomonas aeruginosa strains from cystic fibrosis patients.囊性纤维化患者来源的铜绿假单胞菌菌株的任意引物PCR分型与酶切大片段DNA分型（脉冲场凝胶电泳）比较
J Clin Microbiol. 1995 Aug;33(8):2216-9. doi: 10.1128/jcm.33.8.2216-2219.1995.
2
Random amplified polymorphic DNA typing of Pseudomonas aeruginosa isolates recovered from patients with cystic fibrosis.对从囊性纤维化患者中分离出的铜绿假单胞菌菌株进行随机扩增多态性DNA分型。
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3
PCR-based assay for differentiation of Pseudomonas aeruginosa from other Pseudomonas species recovered from cystic fibrosis patients.基于聚合酶链反应的检测方法，用于区分从囊性纤维化患者中分离出的铜绿假单胞菌与其他假单胞菌属菌种。
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Comparison of ribotyping and genome fingerprinting of Pseudomonas aeruginosa isolates from cystic fibrosis patients.囊性纤维化患者铜绿假单胞菌分离株的核糖体分型与基因组指纹图谱比较
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Comparison of genome fingerprinting with conventional typing methods used on Pseudomonas aeruginosa isolates from cystic fibrosis patients.对来自囊性纤维化患者的铜绿假单胞菌分离株进行基因组指纹分析与传统分型方法的比较。
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Epidemiology and clinical impact of Pseudomonas aeruginosa infection in cystic fibrosis using AP-PCR fingerprinting.运用扩增片段长度多态性聚合酶链反应指纹图谱技术研究铜绿假单胞菌感染在囊性纤维化中的流行病学及临床影响
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Molecular detection of an atypical, highly resistant, clonal Pseudomonas aeruginosa isolate in cystic fibrosis patients.囊性纤维化患者中一株非典型、高度耐药、克隆性铜绿假单胞菌的分子检测。
J Cyst Fibros. 2013 Mar;12(2):141-6. doi: 10.1016/j.jcf.2012.07.007. Epub 2012 Aug 16.

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Pseudomonas aeruginosa in children with cystic fibrosis diagnosed through newborn screening: assessment of clinic exposures and microbial genotypes.通过新生儿筛查诊断的囊性纤维化患儿中的铜绿假单胞菌：临床暴露和微生物基因型评估。
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Pseudomonas aeruginosa cystic fibrosis isolates of similar RAPD genotype exhibit diversity in biofilm forming ability in vitro.相似 RAPD 基因型的铜绿假单胞菌囊性纤维化分离株在体外生物膜形成能力方面表现出多样性。
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本文引用的文献

1
Genetic analysis using random amplified polymorphic DNA markers.使用随机扩增多态性DNA标记进行遗传分析。
Methods Enzymol. 1993;218:704-40. doi: 10.1016/0076-6879(93)18053-f.
2
Antibiotic therapy for chronic infection of pseudomonas in the lung.肺部铜绿假单胞菌慢性感染的抗生素治疗
Annu Rev Med. 1993;44:1-10. doi: 10.1146/annurev.me.44.020193.000245.
3
Random amplified polymorphic DNA assay is less discriminant than pulsed-field gel electrophoresis for typing strains of methicillin-resistant Staphylococcus aureus.对于耐甲氧西林金黄色葡萄球菌菌株的分型，随机扩增多态性DNA分析比脉冲场凝胶电泳的鉴别能力弱。
J Clin Microbiol. 1993 Apr;31(4):982-5. doi: 10.1128/jcm.31.4.982-985.1993.
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Genome macrorestriction analysis of diversity and variability of Pseudomonas aeruginosa strains infecting cystic fibrosis patients.感染囊性纤维化患者的铜绿假单胞菌菌株多样性和变异性的基因组宏观限制性分析
J Clin Microbiol. 1993 Sep;31(9):2320-6. doi: 10.1128/jcm.31.9.2320-2326.1993.
5
Nonmotility and phagocytic resistance of Pseudomonas aeruginosa isolates from chronically colonized patients with cystic fibrosis.从长期定植的囊性纤维化患者中分离出的铜绿假单胞菌菌株的非运动性和吞噬抗性。
Infect Immun. 1994 Feb;62(2):596-605. doi: 10.1128/iai.62.2.596-605.1994.
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RAPD (arbitrary primer) PCR is more sensitive than multilocus enzyme electrophoresis for distinguishing related bacterial strains.随机扩增多态性DNA（任意引物）聚合酶链反应在区分相关细菌菌株方面比多位点酶电泳更为灵敏。
Nucleic Acids Res. 1993 Dec 25;21(25):5930-3. doi: 10.1093/nar/21.25.5930.
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DNA sequence divergence among derivatives of Escherichia coli K-12 detected by arbitrary primer PCR (random amplified polymorphic DNA) fingerprinting.通过任意引物PCR（随机扩增多态性DNA）指纹图谱检测大肠杆菌K-12衍生物之间的DNA序列差异。
J Bacteriol. 1994 Mar;176(6):1673-82. doi: 10.1128/jb.176.6.1673-1682.1994.
8
DNA fingerprinting of medically important microorganisms by use of PCR.利用聚合酶链反应对具有医学重要性的微生物进行DNA指纹分析。
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Pseudomonas aeruginosa corneal ulcer isolates distinguished using the arbitrarily primed PCR DNA fingerprinting method.
Curr Eye Res. 1994 Nov;13(11):783-90. doi: 10.3109/02713689409025132.
10
Genome fingerprinting of Pseudomonas aeruginosa indicates colonization of cystic fibrosis siblings with closely related strains.铜绿假单胞菌的基因组指纹图谱表明，患有囊性纤维化的兄弟姐妹被密切相关的菌株定植。
J Clin Microbiol. 1988 Oct;26(10):1973-7. doi: 10.1128/jcm.26.10.1973-1977.1988.

囊性纤维化患者来源的铜绿假单胞菌菌株的任意引物PCR分型与酶切大片段DNA分型（脉冲场凝胶电泳）比较

Comparison of arbitrarily primed PCR and macrorestriction (pulsed-field gel electrophoresis) typing of Pseudomonas aeruginosa strains from cystic fibrosis patients.

作者信息

Kersulyte D, Struelens M J, Deplano A, Berg D E

机构信息

Department of Molecular Microbiology, Washington University Medical School, St. Louis, Missouri 63110, USA.

出版信息

J Clin Microbiol. 1995 Aug;33(8):2216-9. doi: 10.1128/jcm.33.8.2216-2219.1995.

DOI:10.1128/jcm.33.8.2216-2219.1995
PMID:7559985
原文链接:https://pmc.ncbi.nlm.nih.gov/articles/PMC228372/
Abstract

Arbitrarily primed PCR fingerprinting was carried out on 43 Pseudomonas aeruginosa isolates from cystic fibrosis (CF) patients. Seventeen major groups of strains that coincided with groups also distinguished by macrorestriction (pulsed-field gel electrophoresis) typing were identified. Our results illustrated that a CF patient can carry more than one strain and can carry a given strain for long periods of time and that strains can evolve by changes in drug resistance or other phenotypic traits during long-term colonization. The arbitrarily primed PCR method is recommended for first-pass screening of P. aeruginosa isolates from CF patients, especially when many strains are to be typed, because of its sensitivity and efficiency.

摘要

对来自囊性纤维化（CF）患者的43株铜绿假单胞菌分离株进行了任意引物PCR指纹分析。鉴定出了17个主要菌株组，这些组与通过宏观限制性酶切（脉冲场凝胶电泳）分型所区分的组一致。我们的结果表明，CF患者可能携带不止一种菌株，并且可以长时间携带某一特定菌株，而且菌株在长期定植过程中可能会因耐药性或其他表型特征的变化而发生进化。由于任意引物PCR方法具有灵敏度和高效性，因此推荐用于对CF患者的铜绿假单胞菌分离株进行初步筛选，尤其是在需要对许多菌株进行分型时。