一个编码大麦10.2 kDa光系统I多肽前体的cDNA克隆。
A cDNA clone encoding the precursor for a 10.2 kDa photosystem I polypeptide of barley.
作者信息
Okkels J S, Scheller H V, Jepsen L B, Møller B L
机构信息
Department of Plant Physiology, Royal Veterinary and Agricultural University, Frederiksberg C, Denmark.
出版信息
FEBS Lett. 1989 Jul 3;250(2):575-9. doi: 10.1016/0014-5793(89)80799-9.
Two cDNA clones for the barley photosystem I polypeptide which migrates with an apparent molecular mass of 9.5 kDa on SDS-polyacrylamide gels have been isolated using antibodies and an oligonucleotide probe. The determined N-terminal amino acid sequence for the mature polypeptide confirms the identification of the clones. The 644 base-pair sequence of one of the clones contains one large open reading frame coding for a 14,882 Da precursor polypeptide. The molecular mass of the mature polypeptide is 10 193 Da. The hydropathy plot of the polypeptide shows one membranespanning region with a predicted alpha-helix secondary structure. The gene for the 9.5 kDa polypeptide has been designated PsaH.
利用抗体和寡核苷酸探针，分离出了两个大麦光系统I多肽的cDNA克隆，该多肽在SDS-聚丙烯酰胺凝胶上的表观分子量为9.5 kDa。所测定的成熟多肽的N端氨基酸序列证实了这些克隆的身份。其中一个克隆的644个碱基对序列包含一个大的开放阅读框，编码一个14882 Da的前体多肽。成熟多肽的分子量为10193 Da。该多肽的亲水性图谱显示有一个具有预测α-螺旋二级结构的跨膜区域。9.5 kDa多肽的基因已被命名为PsaH。
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