一个编码大麦10.8 kDa光系统I多肽的cDNA克隆。
A cDNA clone encoding a 10.8 kDa photosystem I polypeptide of barley.
作者信息
Okkels J S, Jepsen L B, Hønberg L S, Lehmbeck J, Scheller H V, Brandt P, Høyer-Hansen G, Stummann B, Henningsen K W, von Wettstein D
机构信息
Department of Plant Physiology, Royal Veterinary and Agricultural University, Frederiksberg C, Denmark.
出版信息
FEBS Lett. 1988 Sep 12;237(1-2):108-12. doi: 10.1016/0014-5793(88)80181-9.
A cDNA clone encoding the barley photosystem I polypeptide which migrates with an apparent molecular mass of 16 kDa on SDS-polyacrylamide gels has been isolated. The 634 bp sequence of this clone has been determined and contains one large open reading frame coding for a 15,457 Da precursor polypeptide. The molecular mass of the mature polypeptide is 10,821 Da. The amino acid sequence of the transit peptide indicates that the polypeptide is routed towards the stroma side of the thylakoid membrane. The hydropathy plot of the polypeptide shows no membrane-spanning regions.
已分离出一个编码大麦光系统I多肽的cDNA克隆，该多肽在SDS-聚丙烯酰胺凝胶上迁移时表观分子量为16 kDa。已确定该克隆的634 bp序列，其中包含一个编码15,457 Da前体多肽的大开放阅读框。成熟多肽的分子量为10,821 Da。转运肽的氨基酸序列表明该多肽被导向类囊体膜的基质侧。该多肽的亲水性图谱显示没有跨膜区域。
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