杆状病毒表达系统中犬细小病毒样颗粒的高产生产。
High-yield production of canine parvovirus virus-like particles in a baculovirus expression system.
作者信息
Jin Hongli, Xia Xiaohong, Liu Bing, Fu Yu, Chen Xianping, Wang Huihui, Xia Zhenqiang
机构信息
Changchun SR Biological Technology Co., LTD, Changchun, 130012, China.
出版信息
Arch Virol. 2016 Mar;161(3):705-10. doi: 10.1007/s00705-015-2719-1. Epub 2015 Dec 14.
An optimized VP2 gene from the current prevalent CPV strain (new CPV-2a) in China was expressed in a baculovirus expression system. It was found that the VP2 proteins assembled into virus-like particles (VLPs) with antigenic properties similar to those of natural CPV and with an especially high hemagglutination (HA) titer (1:2(20)). Dogs intramuscularly or orally immunized with VLPs produced antibodies against CPV with >1:80 hemagglutination inhibition (HI) units for at least 3 months. The CPV VLPs could be considered for use as a vaccine against CPV or as a platform for research on chimeric VLP vaccines against other diseases.
在中国，从当前流行的犬细小病毒（CPV）毒株（新型CPV-2a）中优化得到的VP2基因，在杆状病毒表达系统中进行了表达。结果发现，VP2蛋白组装成了病毒样颗粒（VLPs），其抗原特性与天然CPV相似，且血凝（HA）效价特别高（1:2(20)）。用VLPs进行肌肉注射或口服免疫的犬产生了抗CPV抗体，血凝抑制（HI）单位>1:80，且至少持续3个月。CPV VLPs可被考虑用作抗CPV疫苗，或作为研究针对其他疾病的嵌合VLP疫苗的平台。
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