[不同时间点电针干预对模拟失重大鼠肝脏HSP 70、MDA、SOD及GSH-PX水平的影响]

[Effect of Electroacupuncture Intervention at Different Time-points on Levels of HSP 70, MDA, SOD and GSH-PX of Liver in Rats with Simulated Weightlessness].

作者信息

Song Yan, Zhao Guo-zhen, Zhao Bai-xiao, Ji Bo, Wang De-sheng, Zhang He, Mao Ying-qiu, Zhang Ping, Xu Yong-si, Liu Ya-li, Lu Ya-wen, Dai Jian, Li Ying-hui

出版信息

Zhen Ci Yan Jiu. 2015 Oct;40(5):383-7.

PMID:26669195

Abstract

OBJECTIVE

To observe the effect of acupuncture intervention at different time-points on the activity of superoxide dismutase (SOD), glutathione peroxidase (GSH-PX), the content of malonaldehyde (MDA) and expression of heat shock protein 70 (HSP 70) of liver tissue in rats with simulated weightlessness, so as to explore its mechanism underlying improvement of liver injury in rats with simulated weightlessness.

METHODS

Twenty male Wistar rats were randomly divided into control group, model group, pre-acupuncture group and EA group, 5 rats in each. The model of simulated weightlessness was established by tail suspension for 4 week. One week before the tail suspension, the rats in the pre-acupuncture group were treated with electroacupuncture (EA) at "Shenshu" (BL 23), "Pishu"(BL 20) and "Sanyinjiao" (SP 6) for 30 min before treatment, once a day for 7 days. The rats in the EA group received tail suspension and acupuncture intervention at the same time. EA was applied for 30 min per treatment, once every other day for 14 times. Immunohistochemical staining was used to assay the expression of HSP 70 in the liver tissue. The activities of SOD and GSH-PX and content of MDA in liver tissues were examined by means of colourimetric method. Results Compared with the control group,the expression of HSP 70 and the content of MDA in the liver tissue were increased significantly (P < 0.01), and the activity of SOD and GSH-PX was notably reduced (P < 0.05) in the model group. Compared with the model group, the content of HSP 70 was significantly reduced in the pre-acupuncture group (P < 0.01). There were no significant changes in the levels of SOD, GSH-PX, MDA and HSP 70 in the EA group (P > 0.05). In comparison with the pre-acupuncture group, the activity of GSH-PX was lower (P < 0.05) and the content of MDA was higher (P < 0.05) in the EA group.

CONCLUSION

EA-pretreatment can suppress the increase of liver HSP 70 immunoactivity in rats with simulated weightlessness, being likely to improve the antioxidant ability of liver.

摘要

目的

观察针刺干预不同时间点对模拟失重大鼠肝组织超氧化物歧化酶（SOD）、谷胱甘肽过氧化物酶（GSH-PX）活性、丙二醛（MDA）含量及热休克蛋白70（HSP 70）表达的影响，以探讨其改善模拟失重大鼠肝损伤的机制。

方法

将20只雄性Wistar大鼠随机分为对照组、模型组、针刺预处理组和电针组，每组5只。采用尾部悬吊法建立模拟失重模型4周。在尾部悬吊前1周，针刺预处理组大鼠于治疗前在“肾俞”（BL 23）、“脾俞”（BL 20）和“三阴交”（SP 6）穴行电针治疗30分钟，每天1次，共7天。电针组大鼠在尾部悬吊的同时进行针刺干预。每次电针治疗30分钟，隔天1次，共14次。采用免疫组织化学染色法检测肝组织中HSP 70的表达。采用比色法检测肝组织中SOD、GSH-PX的活性及MDA的含量。结果与对照组比较，模型组肝组织中HSP 70表达及MDA含量显著升高（P<0.01），SOD、GSH-PX活性显著降低（P<0.05）。与模型组比较，针刺预处理组HSP 70含量显著降低（P<0.01）。电针组SOD、GSH-PX、MDA及HSP 70水平无显著变化（P>0.05）。与针刺预处理组比较，电针组GSH-PX活性较低（P<0.05），MDA含量较高（P<0.05）。