Zhang Hei, Zhao Guozhen, Wang Desheng, Zhao Baixiao, Ji Bo, Song Yan, Zhang Ping, Liu Yali, Li Yinghui
Zhongguo Zhen Jiu. 2015 Dec;35(12):1275-9.
To explore the change pattern of hypothalamic-pituitary-adrenal (HPA) axis and related neurotransmitters under simulated weightlessness.
A total of 40 clean-grade male Wistar rats were randomly divided into a normal group, a tail-suspension group, an electroacupuncture (EA) at Neiguan (PC 6) group, an EA at Sanyinjiao (SP 6) group, 10 rats in each group. Rats in the tail-suspension group, EA at "Neiguan" (PC 6) group and EA at "Sanyinjiao" (SP 6) group were treated with tail suspension to simulate weightlessness effect. Rats in the normal group were treated with normal diet. Rats in the tail-suspension group were treated with tail suspension for 28 d. During the time of tail suspension, rats in the EA at "Neiguan" (PC 6) group were treated with EA at "Neiguan" (PC 6), 30 min per treatment, once every two days for 14 treatments, while rats in the EA at "Sanyinjiao" (SP 6) group were treated with EA at "Sanyinjiao" (SP 6), 30 min per treatment, once every two days for 14 treatments. Samples were all collected after 4 weeks. The contents of corticotropin releasing hormone (CRH) , adrenocorticotropic hormone (ACTH), corticosterone (CORT) in as well as 5-hydroxy tryptamine (5-HT), dopamine (DA), norepinephrine (NE) were measured by using radioimmunoassay.
Compared with the normal group, in the tail-suspension group the content of ACTH in pituitary was significantly decreased (P< 0.05), and the content of 5-HT in hypothalamus was significantly decreased (P < 0.01); the content of CRH and 5-HT in hypothalamus was significantly decreased (P < 0.01, P < 0.05) in the EA at "Neiguan" (PC 6) group; the content of CRH and 5-HT in hypothalamus was significantly decreased (P < 0.01), and the content of CORT in serum was significantly decreased (P < 0.05) in the EA at "Sanyinjiao" (SP 6) group. Compared with the tail-suspension group, the content of ACTH in hypothalamus was significantly decreased (P< 0.05) in the EA at "Neiguan" (PC 6) group; the content of CRH, ACTH and CORT was significantly decreased (P < 0.01, P < 0.05) in the EA at "Sanyinjiao" (SP 6) group. Compared with the EA at "Neiguan" (PC 6) group, the content of CORT was decreased (P < 0.05) in the EA at "Sanyinjiao" (SP 6) group.
EA can regulate the content of 5-HT in hypothalamus in tail-suspension rats, inhibit the hyperactivity of the HPA axis, in which EA at "Sanyinjiao" (SP 6) had more significant effects than "Neiguan" (PC 6), but no obvious effects on NE and DA were observed.
探讨模拟失重状态下下丘脑 - 垂体 - 肾上腺（HPA）轴及相关神经递质的变化规律。
将40只清洁级雄性Wistar大鼠随机分为正常组、尾悬吊组、内关（PC 6）电针组、三阴交（SP 6）电针组，每组10只。尾悬吊组、“内关”（PC 6）电针组和“三阴交”（SP 6）电针组大鼠采用尾悬吊法模拟失重效应。正常组大鼠给予正常饮食。尾悬吊组大鼠尾悬吊28天。在尾悬吊期间，“内关”（PC 6）电针组大鼠给予“内关”（PC 6）电针治疗，每次治疗30分钟，每两天1次，共治疗14次；“三阴交”（SP 6）电针组大鼠给予“三阴交”（SP 6）电针治疗，每次治疗30分钟，每两天1次，共治疗14次。4周后采集样本。采用放射免疫法测定促肾上腺皮质激素释放激素（CRH）、促肾上腺皮质激素（ACTH）、皮质酮（CORT）以及5 - 羟色胺（5 - HT）、多巴胺（DA）、去甲肾上腺素（NE）的含量。
与正常组相比，尾悬吊组垂体ACTH含量显著降低（P<0.05），下丘脑5 - HT含量显著降低（P<0.01）；“内关”（PC 6）电针组下丘脑CRH和5 - HT含量显著降低（P<0.01，P<0.05）；“三阴交”（SP 6）电针组下丘脑CRH和5 - HT含量显著降低（P<0.01），血清CORT含量显著降低（P<0.05）。与尾悬吊组相比，“内关”（PC 6）电针组下丘脑ACTH含量显著降低（P<0.05）；“三阴交”（SP 6）电针组CRH、ACTH和CORT含量显著降低（P<0.01，P<0.05）。与“内关”（PC 6）电针组相比，“三阴交”（SP 6）电针组CORT含量降低（P<0.05）。
电针可调节尾悬吊大鼠下丘脑5 - HT含量，抑制HPA轴的亢进，其中“三阴交”（SP 6）电针的作用比“内关”（PC 6）电针更显著，但对NE和DA无明显影响。
