State Key Laboratory of Electroanalytical Chemistry, Changchun Institute of Applied Chemistry, Chinese Academy of Sciences , 5625 Renmin Street, Changchun, Jilin 130022, P.R. China.
University of the Chinese Academy of Sciences, Chinese Academy of Sciences , No. 19A Yuquanlu, Beijing 100049, P.R. China.
Anal Chem. 2016 Jan 19;88(2):1123-7. doi: 10.1021/acs.analchem.5b04304. Epub 2015 Dec 24.
Both a wireless electrochemiluminescence (ECL) electrode microarray chip and the dramatic increase in ECL by embedding a diode in an electromagnetic receiver coil have been first reported. The newly designed device consists of a chip and a transmitter. The chip has an electromagnetic receiver coil, a mini-diode, and a gold electrode array. The mini-diode can rectify alternating current into direct current and thus enhance ECL intensities by 18 thousand times, enabling a sensitive visual detection using common cameras or smart phones as low cost detectors. The detection limit of hydrogen peroxide using a digital camera is comparable to that using photomultiplier tube (PMT)-based detectors. Coupled with a PMT-based detector, the device can detect luminol with higher sensitivity with linear ranges from 10 nM to 1 mM. Because of the advantages including high sensitivity, high throughput, low cost, high portability, and simplicity, it is promising in point of care testing, drug screening, and high throughput analysis.
首次报道了一种无线电化学发光（ECL）电极微阵列芯片和通过在电磁接收器线圈中嵌入二极管使 ECL 显著增强的方法。新设计的装置由芯片和发射器组成。芯片具有电磁接收器线圈、微型二极管和金电极阵列。微型二极管可以将交流电整流为直流电，从而将 ECL 强度增强 18000 倍，使得使用普通相机或智能手机作为低成本探测器进行灵敏的视觉检测成为可能。使用数码相机检测过氧化氢的检测限可与基于光电倍增管（PMT）的检测器相媲美。与基于 PMT 的检测器结合使用时，该装置可以以 10 nM 至 1 mM 的线性范围检测到更高灵敏度的鲁米诺。由于具有高灵敏度、高通量、低成本、高便携性和简单性等优点，该装置在即时检测、药物筛选和高通量分析方面具有广阔的应用前景。
