Jouan A, Coulibaly I, Lena P, Sarthou J L, Leguenno B, Meegan J, Ksiazek T
Institut Pasteur, Dakar.
Res Virol. 1989 Mar-Apr;140(2):169-73. doi: 10.1016/s0923-2516(89)80095-0.
In order to collect epidemiological data about the Rift Valley fever epidemic in Mauritania, we decided to use the filter paper method. The mean recovery level of specific antibodies from filter paper, tested using an immunoenzymatic method, is around one fourth. Taking the mean haematocrit into account, we estimated the extract square with a 1/300 dilution. This method was very useful for epidemiological studies, we observed few patient refusals, but it is necessary to know the exact specificity of the antibodies.
为了收集毛里塔尼亚裂谷热疫情的流行病学数据,我们决定采用滤纸法。使用免疫酶法检测,滤纸上特异性抗体的平均回收率约为四分之一。考虑到平均血细胞比容,我们估计以1/300稀释度提取的样本。该方法对流行病学研究非常有用,我们观察到很少有患者拒绝,但有必要了解抗体的确切特异性。