Knauert F K, Meegan J M, Jouan A, Ksiazek T G, Le Guenno B, Sarthou J L, Peters C J, Digoutte J P
Disease Assessment Division, United States Army Medical Research Institute of Infectious Diseases, Ft. Detrick, Frederick, MD 21701-5011.
Res Virol. 1989 Jan-Feb;140(1):47-57. doi: 10.1016/s0923-2516(89)80084-6.
The Rift Valley fever virus (RVFV) epidemic that occurred in southern Mauritania during the 1987 rainy season provided a unique opportunity to test and evaluate a recently developed, M-segment-specific, nucleic acid filter hybridization assay on a large collection of infected human serum samples. It afforded the opportunity to compare the procedure with two other methods for detecting virus: virus isolation and antigen detection by ELISA. The filter hybridization procedure employed a polyethylene-glycol-precipitation and proteinase-K-digestion sample treatment step developed specifically for preparing serum samples for hybridization. The procedure was less sensitive for detecting RVFV in the Mauritanian human viremic samples than in sera from experimentally infected monkeys used to evaluate this procedure. It was also less sensitive than an antigen detection procedure used to test the Mauritanian samples. However, we were able to detect virus RNA in a significant proportion of the virus-isolation-positive samples. Advances in sample preparation, labelling and detection procedures, and hybridization methods will improve the sensitivity, precision and ease of use of this assay and increase its value as a diagnostic tool.
1987年雨季在毛里塔尼亚南部发生的裂谷热病毒（RVFV）疫情提供了一个独特的机会，可在大量受感染的人类血清样本上测试和评估一种最近开发的、针对M片段的核酸滤膜杂交检测方法。这使得能够将该方法与另外两种检测病毒的方法进行比较：病毒分离和通过酶联免疫吸附测定（ELISA）进行抗原检测。滤膜杂交方法采用了专门为制备用于杂交的血清样本而开发的聚乙二醇沉淀和蛋白酶K消化样本处理步骤。该方法在检测毛里塔尼亚人类病毒血症样本中的RVFV时，不如在用于评估该方法的实验感染猴子的血清中敏感。它也不如用于检测毛里塔尼亚样本的抗原检测方法敏感。然而，我们能够在相当一部分病毒分离阳性样本中检测到病毒RNA。样本制备、标记和检测程序以及杂交方法的进步将提高该检测方法的灵敏度、精密度和易用性，并增加其作为诊断工具的价值。
