Wallingford R A, Ewing A G
Pennsylvania State University, University Park.
Adv Chromatogr. 1989;29:1-76.
The combined techniques that comprise capillary electrophoresis are under rapid development for a wide range of molecules and sample types. The capillary format provides fast, highly efficient separations and makes automation possible. At present, systematic approaches to separation methodology are still in the development stages and better detection schemes need to be developed. There is no doubt that electrophoresis in a capillary tube will take its place as a substitute for HPLC in specific applications. This is especially apparent when analysis time, efficiency, or simplicity is of greatest concern. Capillary electrophoresis will prove extremely useful for separations of nucleotides and proteins for analytical purposes in biotechnology. Finally, the ability to use ultrasmall-volume samples makes capillary electrophoresis ideal for the development of separations-based sensors for the analysis of microenvironments.
构成毛细管电泳的组合技术正针对广泛的分子和样品类型迅速发展。毛细管形式提供了快速、高效的分离，并使自动化成为可能。目前，分离方法的系统方法仍处于发展阶段，需要开发更好的检测方案。毫无疑问，毛细管中的电泳将在特定应用中取代高效液相色谱。当分析时间、效率或简单性最为重要时，这一点尤为明显。毛细管电泳对于生物技术中用于分析目的的核苷酸和蛋白质的分离将证明极为有用。最后，使用超小体积样品的能力使毛细管电泳成为开发用于微环境分析的基于分离的传感器的理想选择。
