Landers J P, Oda R P, Spelsberg T C, Nolan J A, Ulfelder K J
Department of Biochemistry and Molecular Biology, Mayo Clinic, Rochester, MN 55905.
Biotechniques. 1993 Jan;14(1):98-111.
Capillary electrophoresis (CE) is a versatile microanalytical technique that has gained much attention, particularly from those working with biologically active molecules. Its appealing characteristics include unprecedented sensitivity and the ability for automating the rapid electrophoretic separation of a number of low-volume samples in a reproducible manner, with relatively short analysis times. The picomole-femtomole (10(-12)-10(-15) mol) sensitivity of UV-CE has been enhanced tremendously by the interfacing of detection systems such as laser-induced fluorescence, which has extended the sensitivity into the attomole-zeptomole (10(-18)-10(-21) mol) range. Fluorescence detection has shown great potential for the CE analysis of a wide range of biomolecules including peptides, proteins and DNA. CE research and development has taken on directions focused primarily on improving detection, understanding and exploiting the basic chemistry of CE and devising new applications.
毛细管电泳（CE）是一种用途广泛的微分析技术，已备受关注，尤其是受到那些从事生物活性分子研究的人员的关注。其吸引人的特性包括前所未有的灵敏度，以及能够以可重复的方式自动快速电泳分离多个小体积样品，且分析时间相对较短。通过诸如激光诱导荧光等检测系统的联用，紫外毛细管电泳的皮摩尔-飞摩尔（10⁻¹² - 10⁻¹⁵摩尔）灵敏度得到了极大提高，将灵敏度扩展到了阿托摩尔-仄托摩尔（10⁻¹⁸ - 10⁻²¹摩尔）范围。荧光检测在包括肽、蛋白质和DNA在内的多种生物分子的毛细管电泳分析中显示出巨大潜力。毛细管电泳的研究与开发主要朝着改进检测、理解和利用毛细管电泳的基础化学以及设计新应用的方向发展。
