Lie T
Scand J Dent Res. 1977 May;85(4):217-31. doi: 10.1111/j.1600-0722.1977.tb00556.x.
The morphology of pellicle formation was studied using correlated transmission and scanning electron microscopy. Pellicle developed on hydroxyapatite/epoxy resin splints attached to the buccal surfaces of molars and premolars in six young individuals. Splint segments were removed at intervals of 2, 4, 6, 12, 24, and 48 h. Measurements of pellicle thickness revealed that during the first 12 h considerably more material formed on the apatite surfaces than on the epoxy resin areas of the splints. This difference was equalized in 24- and 48-h specimens. Three types of pellicles could be distinguished on the basis of morphologic criteria. A globular pellicle, characterized by a consistent presence of globules of varying size and configuration, seemed to predominate in most subjects. These globules could not be mistaken for bacteria due to their dimensions, contour, and lack of cell walls. A fibrillar pellicle consisted of 3- to 7-nm-wide fibrils and frequently included some finely globular particles. A granular pellicle displayed a relatively even surface contour and did not exhibit globular or fibrillar structures. In contrast to the two other types, the granular pellicle contained distinct laminations. Apparently, the varying morphology of the early pellicle is a reflection of its complex chemical composition.
使用相关的透射电子显微镜和扫描电子显微镜研究了薄膜形成的形态。在六个年轻个体的磨牙和前磨牙颊面附着的羟基磷灰石/环氧树脂夹板上形成了薄膜。夹板段每隔2、4、6、12、24和48小时取出一次。薄膜厚度测量显示，在最初的12小时内，磷灰石表面形成的物质比夹板的环氧树脂区域多得多。在24小时和48小时的标本中，这种差异趋于平衡。根据形态学标准可区分出三种类型的薄膜。球状薄膜的特征是始终存在大小和形态各异的小球，在大多数受试者中似乎占主导地位。由于其尺寸、轮廓和缺乏细胞壁，这些小球不会被误认为是细菌。纤维状薄膜由3至7纳米宽的纤维组成，并且经常包含一些细小的球状颗粒。颗粒状薄膜呈现出相对均匀的表面轮廓，并且没有球状或纤维状结构。与其他两种类型不同，颗粒状薄膜包含明显的分层。显然，早期薄膜形态的变化反映了其复杂的化学成分。
