Hannig M
Clinic of Operative Dentistry and Periodontology, Christian-Albrechts-University of Kiel, Germany.
Eur J Oral Sci. 1997 Oct;105(5 Pt 1):422-33. doi: 10.1111/j.1600-0722.1997.tb02139.x.
This electron microscopic investigation was performed to examine the ultrastructure of the in vivo formed salivary pellicle on 15 different dental materials. Test pieces of amalgam alloys, casting alloys, titanium, ceramics, resins, composite resins, glass polyalkenoate cement, and bovine enamel were attached to the buccal and lingual surfaces of the upper first molars in three adults using removable intraoral splints. The splints were carried over periods of 2 and 6 h. Pellicle-like structures could be identified on all tested material surfaces by transmission electron microscopic analysis. The pellicle layer showed a high degree of similarity on different kinds of surfaces with regard to the ultrastructural appearance. However, distinct differences could be detected in the ultrastructural pattern and thickness of the pellicle layer formed on buccally and lingually mounted specimens. Pellicles adsorbed on buccally carried test pieces were characterized by a heterogeneous, globular appearance and a thickness ranging from 500 to 1,000 nm after 6 h. In contrast, all lingually mounted test pieces were covered by a granular pellicle of about 100 nm thickness after 6 h. It is concluded that the ultrastructural pattern and extent of salivary pellicle formation are influenced by locally available salivary biopolymers and locally effective shearing forces rather than by material-dependent parameters.
进行这项电子显微镜研究是为了检查在15种不同牙科材料上体内形成的唾液薄膜的超微结构。使用可摘除的口腔内夹板将汞合金、铸造合金、钛、陶瓷、树脂、复合树脂、玻璃聚烯酸酯粘固粉和牛牙釉质的试件附着在三名成年人上颌第一磨牙的颊面和舌面。夹板佩戴2小时和6小时。通过透射电子显微镜分析，在所有测试材料表面均可识别出薄膜状结构。就超微结构外观而言，薄膜层在不同类型的表面上表现出高度相似性。然而，在颊面和舌面安装的试件上形成的薄膜层的超微结构模式和厚度存在明显差异。6小时后，吸附在颊面携带的试件上的薄膜具有异质、球状外观，厚度在500至1000纳米之间。相比之下，6小时后，所有舌面安装的试件都被一层厚度约为100纳米的颗粒状薄膜覆盖。得出的结论是，唾液薄膜形成的超微结构模式和程度受局部可用的唾液生物聚合物和局部有效剪切力的影响，而不是受材料相关参数的影响。
