Sipaúba-Tavares L H, Berchielli-Morais F A, Scardoeli-Truzzi B
Limnology and Plankton Production Laboratory, Aquaculture Center, Universidade Estadual Paulista, Jaboticabal, SP, Brazil.
Braz J Biol. 2015 Nov;75(4):796-803. doi: 10.1590/1519-6984.23013. Epub 2015 Nov 10.
Current study investigates the effect of two alternative media NPK (20-5-20) fertilizer and NPK plus macrophyte (M+NPK) compared to the commercial medium (WC) under growth rate and physiological parameters in batch culture mode (2-L), and verifies whether the use of fertilizer (NPK) and macrophyte (Eichhornia crassipes) would be a good tool for Haematococcus pluvialis culture in the laboratory. The highest number of cells of H. pluvialis has been reported in NPK medium (5.4 × 105 cells.mL-1) on the 28th day, and in the M+NPK and WC media (4.1 × 105 cells.mL-1 and 2.1 × 105 cells.mL-1) on the 26th day, respectively. Chlorophyll-a contents were significantly higher (p<0.05) in NPK medium (41-102 µg.L-1) and lower in WC and M+NPK media (14-61 µg.L-1). The astaxanthin content was less than 0.04 mg.L-1. Production cost of 10-L of H. pluvialis was low in all media, and NPK and M+NPK media had a cost reduction of 65% and 82%, respectively when compared with commercial medium (WC). The use of a medium based on commercial fertilizer and macrophyte (E. crassipes) produced a new medium formulation that proved to be efficient, at least in batch culture mode, in promoting high density culture of H. pluvialis. NPK and macrophyte (E. crassipes) medium seems to be an adequate alternative to replace the conventional one (WC).
当前研究调查了在分批培养模式（2升）下，两种替代培养基（NPK（20-5-20）肥料和NPK加大型植物（M+NPK））与商业培养基（WC）相比，对生长速率和生理参数的影响，并验证肥料（NPK）和大型植物（凤眼莲）的使用是否是实验室中雨生红球藻培养的良好工具。据报道，在第28天，雨生红球藻在NPK培养基中的细胞数量最高（5.4×10⁵个细胞·mL⁻¹），在第26天，在M+NPK和WC培养基中的细胞数量分别为（4.1×10⁵个细胞·mL⁻¹和2.1×10⁵个细胞·mL⁻¹）。叶绿素a含量在NPK培养基中显著更高（p<0.05）（41-102μg·L⁻¹），在WC和M+NPK培养基中较低（14-61μg·L⁻¹）。虾青素含量低于0.04mg·L⁻¹。在所有培养基中，10升雨生红球藻的生产成本都很低，与商业培养基（WC）相比，NPK和M+NPK培养基的成本分别降低了65%和82%。使用基于商业肥料和大型植物（凤眼莲）的培养基产生了一种新的培养基配方，该配方至少在分批培养模式下，被证明在促进雨生红球藻高密度培养方面是有效的。NPK和大型植物（凤眼莲）培养基似乎是替代传统培养基（WC）的合适选择。
