Colusse Guilherme Augusto, Mendes Carlos Rafael Borges, Duarte Maria Eugênia Rabello, Carvalho Julio Cesar de, Noseda Miguel Daniel
Postgraduate Program in Bioprocess Engineering and Biotechnology, Federal University of Paraná, Av. Cel. Francisco H. dos Santos, 100, CEP 81531-990, PO BOX 19011, Curitiba, Brazil.
Biochemistry and Molecular Biology Department, Federal University of Paraná, Av. Cel. Francisco H. dos Santos, 100, CEP 81531-980, PO BOX 19046, Curitiba, Brazil.
Biotechnol Rep (Amst). 2020 Jul 24;27:e00508. doi: 10.1016/j.btre.2020.e00508. eCollection 2020 Sep.
Microalgal cultivation system depends on optimal parameters obtained from laboratory conditions to overcome high-value compounds production and decrease cost. In this study, the laboratory-scale cultivation of was performed to explore the cost and biochemical effects on biomass cultivated using F/2, Conway, and Johnson media. cultivation was monitored by cell counting, dry biomass measurements and biochemical analyses. Photosynthetic pigment profiles were identified and quantified through high-performance liquid chromatography (HPLC) whereas economic evaluation was based on media cost. Cellular growth parameters were significantly improved by Conway medium, whereas maximum accumulation of pigments, especially violaxanthin (0.855 mg g) and chlorophyll (14.255 mg g) were observed when Johnson medium was used. Conway-biomass showed 43 % of total lipid content. This value represents 1.5 and 1.4 times higher than lipid content found in Johnson- and F/2-biomass, respectively. Furthermore, media cost presented a variation of US$1.17-49.62 for each 10 L of media mainly due to NaNO, KNO and cyanocobalamin supplementation. Thus, biomass production showed a cost variation of US$4.64-301.61 per kg on a dry weight basis. These outcomes emphasize the suitability of laboratory parameters and cost comparison among biomass produced by different media.
微藻培养系统依赖于从实验室条件获得的最佳参数，以实现高价值化合物的生产并降低成本。在本研究中，进行了实验室规模的[微藻名称未给出]培养，以探索使用F/2培养基、康威培养基和约翰逊培养基培养生物量的成本和生化影响。通过细胞计数、干生物量测量和生化分析对[微藻名称未给出]培养进行监测。通过高效液相色谱法（HPLC）鉴定并定量光合色素谱，而经济评估基于培养基成本。康威培养基显著改善了细胞生长参数，而使用约翰逊培养基时观察到色素的最大积累，尤其是紫黄质（0.855 mg/g）和叶绿素[此处叶绿素具体种类未明确]（14.255 mg/g）。康威生物量显示总脂质含量为43%。该值分别比约翰逊生物量和F/2生物量中的脂质含量高1.5倍和1.4倍。此外，由于添加了硝酸钠、硝酸钾和钴胺素，每10升培养基的成本在1.17 - 49.62美元之间变化。因此，以干重计，生物量生产的成本在每千克4.64 - 301.61美元之间变化。这些结果强调了实验室参数的适用性以及不同培养基生产的生物量之间的成本比较。
