Moreira Fernanda Pedrotti, Medeiros João Ricardo Carvalho, Lhullier Alfredo Cardoso, Souza Luciano Dias de Mattos, Jansen Karen, Portela Luis Valmor, Lara Diogo R, da Silva Ricardo Azevedo, Wiener Carolina David, Oses Jean Pierre
Translational Science on Brain Disorders, Department of Health and Behavior, Center of Life and Health Sciences, Catholic University of Pelotas, Pelotas, Brazil.
Psychology School, Center of Life and Health Sciences, Catholic University of Pelotas, Pelotas, Brazil.
Drug Alcohol Depend. 2016 Jan 1;158:181-5. doi: 10.1016/j.drugalcdep.2015.11.024. Epub 2015 Nov 26.
Cocaine abuse is capable of activating the innate immune system in the CNS resulting in deregulation of homeostasis between pro and antiinflammatory cytokines. The aim of this study was to investigate serum levels of pro and antiinflammatory cytokines, IL-6 and IL-10 respectively, in cocaine users from a young population-based sample.
This is a case-control study nested in a cross-sectional population-based survey, with individuals of 18 and 35 years old. Two groups were selected: 24 healthy controls and 12 subjects who reported cocaine use. Serum IL-6 and IL-10 were measured by ELISA using a commercial kit.
There was a statistically significant increase in IL-6 (p=0.037) and decrease in IL-10 (p=0.007) serum levels, between cocaine users and the control group. There was also an increase in the ratio IL-6/IL-10 (p=0.013) among cocaine users individuals, when compared to the control group.
Our results suggest that cocaine users showed an activation of the immune system when compared a control group, demonstrating a disruption in the balance of pro and antiinflammatory cytokines. Thus, peripheral cytokines may represent a putative biomarkers for cocaine users, contributing to the development of diagnosis and effective treatments.
可卡因滥用能够激活中枢神经系统中的先天免疫系统，导致促炎细胞因子和抗炎细胞因子之间的稳态失调。本研究的目的是调查来自年轻人群样本的可卡因使用者血清中促炎细胞因子和抗炎细胞因子（分别为白细胞介素-6和白细胞介素-10）的水平。
这是一项嵌套在基于人群的横断面调查中的病例对照研究，研究对象为18至35岁的个体。选取了两组：24名健康对照者和12名报告使用过可卡因的受试者。使用商用试剂盒通过酶联免疫吸附测定法（ELISA）测量血清白细胞介素-6和白细胞介素-10。
可卡因使用者和对照组之间，血清白细胞介素-6水平有统计学意义的升高（p=0.037），白细胞介素-10水平有统计学意义的降低（p=0.007）。与对照组相比，可卡因使用者个体中白细胞介素-6/白细胞介素-10的比值也有所升高（p=0.013）。
我们的结果表明，与对照组相比，可卡因使用者的免疫系统被激活，这表明促炎细胞因子和抗炎细胞因子的平衡受到破坏。因此，外周细胞因子可能是可卡因使用者的一种潜在生物标志物，有助于诊断和有效治疗方法的开发。
