Yabe Yutaka, Hagiwara Yoshihiro, Tsuchiya Masahiro, Honda Masahito, Hatori Kouki, Sonofuchi Kazuaki, Kanazawa Kenji, Koide Masashi, Sekiguchi Takuya, Itaya Nobuyuki, Itoi Eiji
Department of Orthopaedic Surgery, Graduate School of Medicine, Tohoku University, Sendai, Japan.
Tohoku Fukushi University, Sendai, Japan.
J Orthop Res. 2016 Jul;34(7):1241-7. doi: 10.1002/jor.23130. Epub 2015 Dec 30.
Elastic fibers and proteoglycans are major components of the extracellular matrix and their changes have been reported in some pathological conditions. Further, recent studies have indicated that some glycosaminoglycans and proteoglycans inhibit elastic fiber assembly. The purpose of this study was to investigate changes of the elastic fibers and proteoglycans in the ligamentum flavum and analyze their relationships to thickening of the ligamentum flavum from lumbar spinal canal stenosis (LSCS). Ligamentum flavum samples were collected from 20 patients with LSCS (thickened flavum group) and 10 patients with lumbar disc herniation (non-thickened flavum group) as a control. Elastica-Masson staining and alcian blue staining were used to compare the relationship between the changes in the elastic fibers and proteoglycans. Gene and protein expressions of the elastic fibers and proteoglycans were analyzed by quantitative reverse transcription polymerase chain reaction and immunohistochemistry. Histological changes indicated that proteoglycans mainly increased on the dorsal side of the ligamentum flavum in accordance with the decreased elastic fibers in the thickened flavum group. The gene and protein expressions of fibrillin-2 and DANCE were significantly lower and decorin, lumican, osteoglycin, and versican were significantly higher in the thickened flavum group. Our study shows that elastic fibers decrease and proteoglycans increase in the thickened ligamentum flavum. Decreased gene expression of elastogenesis and disrupted elastic fiber assembly caused by increased proteoglycans may lead to a loss of elasticity in the thickened ligamentum flavum. Decreased elasticity may cause buckling of the tissue, which leads to thickening of the ligamentum flavum. © 2015 Orthopaedic Research Society. Published by Wiley Periodicals, Inc. J Orthop Res 34:1241-1247, 2016.
弹性纤维和蛋白聚糖是细胞外基质的主要成分，在某些病理状况下其会发生变化。此外，最近的研究表明，一些糖胺聚糖和蛋白聚糖会抑制弹性纤维组装。本研究的目的是调查黄韧带中弹性纤维和蛋白聚糖的变化，并分析它们与腰椎管狭窄症（LSCS）导致的黄韧带增厚之间的关系。从20例LSCS患者（黄韧带增厚组）和10例腰椎间盘突出症患者（黄韧带未增厚组）收集黄韧带样本作为对照。采用弹力纤维-马松染色和阿尔辛蓝染色来比较弹性纤维和蛋白聚糖变化之间的关系。通过定量逆转录聚合酶链反应和免疫组织化学分析弹性纤维和蛋白聚糖的基因及蛋白表达。组织学变化表明，在增厚的黄韧带组中，随着弹性纤维减少，蛋白聚糖主要在黄韧带背侧增加。在增厚的黄韧带组中，原纤蛋白-2和DANCE的基因及蛋白表达显著降低，而核心蛋白聚糖、光蛋白聚糖、骨形成蛋白聚糖和多功能蛋白聚糖的表达显著升高。我们的研究表明，增厚的黄韧带中弹性纤维减少而蛋白聚糖增加。蛋白聚糖增加导致弹性生成的基因表达降低和弹性纤维组装破坏，可能会致使增厚的黄韧带失去弹性。弹性降低可能会导致组织弯曲，进而导致黄韧带增厚。©2015骨科研究学会。由威利期刊公司出版。《矫形外科学研究》34:1241 - 1247，2016年。
