Sirotkin Alexander V, Kardošová Diana, Alwasel Saleh Hamad, Harrath Abdel Halim
Constantine The Philosopher University in Nitra, Nitra, Slovakia; Research Institute of Animal Production Nitra, Lužianky, Slovakia; King Saud University, Department of Zoology, College of Science, Riyadh, Saudi Arabia.
Constantine The Philosopher University in Nitra, Nitra, Slovakia.
Reprod Biol. 2015 Dec;15(4):257-60. doi: 10.1016/j.repbio.2015.07.004. Epub 2015 Aug 4.
The effects of neuropeptide Y (NPY; 0, 10, 100 and 1000 ng/mL) on the expression of PCNA, bax and p53 were examined by immunocytochemistry in porcine luteinized granulosa cells. NPY inhibited proliferation as well as promoted apoptosis and accumulation of p53 in the cells. This is the first report to demonstrate the direct action of NPY on ovarian cell proliferation and apoptosis. The results of the study suggest that the effect is mediated by transcription factor p53.
采用免疫细胞化学方法，检测了神经肽Y（NPY；0、10、100和1000 ng/mL）对猪黄体化颗粒细胞中增殖细胞核抗原（PCNA）、bax和p53表达的影响。NPY抑制细胞增殖，促进细胞凋亡及p53在细胞中的蓄积。这是首篇证明NPY对卵巢细胞增殖和凋亡具有直接作用的报道。该研究结果提示，此效应由转录因子p53介导。
