Cheng Tegan L, Schindeler Aaron, Little David G
Orthopaedic Research and Biotechnology Unit, The Children's Hospital at Westmead, Locked Bag 4001, Westmead, Sydney, New South Wales, 2145, Australia.
Discipline of Paediatrics and Child Health, Sydney Medical School, University of Sydney, Sydney, Australia.
J Orthop Res. 2016 Jul;34(7):1168-76. doi: 10.1002/jor.23131. Epub 2016 Jan 8.
Human bone morphogenetic proteins (BMPs) are an alternative to bone graft for the treatment of high-energy open fractures. The standard delivery system for BMP-2 is a porous collagen sponge, but we have previously found that the biocompatible, high viscosity carrier, Sucrose acetate isobutyrate (SAIB) is an effective and potentially less invasive alternative. The efficacy of SAIB as a BMP-2 delivery system was examined in an open fracture model featuring a femoral osteotomy with periosteal stripping in 9-week-old male Sprague Dawley rats. SAIB containing BMP-2 (SAIB/BMP-2) was delivered into the fracture site during surgery and an additional group was further co-treated with zoledronic acid and hydroxyapatite nanoparticles (SAIB/BMP-2/HA/ZA). These were compared to untreated fractures and SAIB carrier alone (negative controls), and BMP-2 loaded collagen sponge (positive control). The rate of radiographic union and the biomechanical properties of the healed fractures were compared after 6-week. Untreated and SAIB-treated fractures showed poor repair, with 53% and 64%, respectively, not bridged at 6 week. In contrast, collagen/BMP-2, SAIB/BMP-2, and SAIB/BMP-2/HA/ZA showed significantly increased union (100%, 100%, and 94%, respectively, p < 0.05). Four-point bend testing revealed that collagen/BMP-2 and SAIB/BMP-2/HA/ZA restored the strength of fractured femora to that of intact femora by 6 week, whereas untreated and SAIB remained less than intact controls by 60% and 67%, respectively (p < 0.05). Overall, the SAIB/BMP-2/HA/ZA formulation was comparable to BMP-2 infused collagen sponge in terms of promoting open fractures repair, but with the additional potential for less invasive delivery. © 2015 Orthopaedic Research Society. Published by Wiley Periodicals, Inc. J Orthop Res 34:1168-1176, 2016.
人骨形态发生蛋白（BMPs）是治疗高能开放性骨折的骨移植替代物。BMP - 2的标准递送系统是多孔胶原海绵，但我们之前发现，生物相容性好、高粘度的载体——蔗糖乙酸异丁酸酯（SAIB）是一种有效且潜在侵入性较小的替代物。在9周龄雄性斯普拉格 - 道利大鼠的股骨截骨术并骨膜剥离的开放性骨折模型中，研究了SAIB作为BMP - 2递送系统的疗效。手术期间将含BMP - 2的SAIB（SAIB/BMP - 2）递送至骨折部位，另一组进一步用唑来膦酸和羟基磷灰石纳米颗粒联合治疗（SAIB/BMP - 2/HA/ZA）。将这些与未治疗的骨折和单独的SAIB载体（阴性对照）以及负载BMP - 2的胶原海绵（阳性对照）进行比较。6周后比较放射学骨愈合率和愈合骨折的生物力学性能。未治疗和SAIB治疗组的骨折修复较差，6周时分别有53%和64%未桥接。相比之下，胶原/BMP - 2、SAIB/BMP - 2和SAIB/BMP - 2/HA/ZA组的骨愈合率显著提高（分别为100%、100%和94%，p < 0.05）。四点弯曲试验显示，胶原/BMP - 2和SAIB/BMP - 2/HA/ZA组在6周时将骨折股骨的强度恢复至完整股骨的强度，而未治疗组和SAIB组分别比完整对照组低60%和67%（p < 0.05）。总体而言，在促进开放性骨折修复方面，SAIB/BMP - 2/HA/ZA配方与注入BMP - 2的胶原海绵相当，但具有侵入性较小递送的额外潜力。©2015骨科研究协会。由威利期刊公司出版。《矫形外科学研究》34:1168 - 1176，2016年。
