a Department of Prosthodontics , Stomatological Hospital of Chongqing Medical University , Chongqing , China.
b Chongqing Key Laboratory of Oral Diseases and Biomedical Sciences , Chongqing , China.
Drug Deliv. 2019 Dec;26(1):137-146. doi: 10.1080/10717544.2019.1568620.
The burst release of active osteogenic factors, which is not beneficial to osteogenesis, is commonly encountered in bone tissue engineering. The aims of this study were to prepare naringin-loaded microsphere/sucrose acetate isobutyrate (Ng-m-SAIB) hybrid depots, reduce the burst release of naringin (Ng), and improve osteogenesis. The morphology and size distributions of electrosprayed Ng-microspheres were characterized by scanning electron microscopy (SEM). The Ng-microspheres and Ng-m-SAIB depots were characterized by Fourier transform infrared spectroscopy (FTIR) and in vitro release studies. In vitro osteoblast-microsphere interactions and in vivo osteogenesis were assessed after implantation of Ng-m-SAIB depots. The addition of sucrose acetate isobutyrate (SAIB) to monodisperse Ng-microspheres did not cause a change in the chemical structure. The performances of the microspheres in osteoblast-microsphere interactions were better when the naringin content was 4% than when it was at 2% and 6%. On the first day following the loading of Ng-microspheres (2%, 4%, and 6%) into SAIB depots, the burst release was reduced dramatically from 70.9% to 6.3%, 73.1% to 7.2%, and 73.9% to 9.9%, respectively. In addition, after 8 weeks, the new bone formation rate in the calvarial defects of SD rats receiving Ng-m-SAIB was 53.1% compared to 21.2% for the control group and 16.1% for the microsphere-SAIB group. These results demonstrated that Ng-m-SAIB hybrid depots may have promise in bone regeneration applications.
在骨组织工程中，经常会遇到活性成骨因子的爆发释放，这不利于成骨。本研究旨在制备柚皮苷载药微球/醋酸丁酸蔗糖酯（Ng-m-SAIB）混合储库，减少柚皮苷（Ng）的爆发释放，并改善成骨作用。通过扫描电子显微镜（SEM）对电纺 Ng 微球的形态和粒径分布进行了表征。通过傅里叶变换红外光谱（FTIR）和体外释放研究对 Ng 微球和 Ng-m-SAIB 储库进行了表征。植入 Ng-m-SAIB 储库后，评估了 Ng-m-SAIB 储库对成骨细胞-微球相互作用和体内成骨的影响。在单分散 Ng 微球中添加醋酸丁酸蔗糖酯（SAIB）不会引起化学结构的变化。当柚皮苷含量为 4%时，微球在成骨细胞-微球相互作用中的性能优于 2%和 6%。在将 Ng 微球（2%、4%和 6%）载入 SAIB 储库的第一天，突释从 70.9%分别显著降低至 6.3%、73.1%至 7.2%和 73.9%至 9.9%。此外，在 8 周后，接受 Ng-m-SAIB 的 SD 大鼠颅骨缺损中的新骨形成率为 53.1%，而对照组为 21.2%，微球-SAIB 组为 16.1%。这些结果表明，Ng-m-SAIB 混合储库可能在骨再生应用中具有潜力。
