Akiyama K, Inoue K, Senshu T
Department of Biochemistry, Tokyo Metropolitan Institute of Gerontology, Japan.
Endocrinology. 1989 Sep;125(3):1121-7. doi: 10.1210/endo-125-3-1121.
We have performed an immunocytochemical study of peptidylarginine deiminase (EC 3.5.3.15) in rat pituitaries. Male and female rat pituitaries were fixed by brief transcardial perfusion of Bouin Hollande Sublimate solution. Immunoperoxidase staining of cryosections obtained from female pituitaries with an antiserum to rat skeletal muscle peptidylarginine deiminase yielded specific staining of numerous cells in the pars distalis. In contrast, few immunopositive cells were noted in the pars distalis of male pituitaries. At least some of the immunopositive cells in female pars distalis were also stained with an antiserum to rat PRL. No such coincidence of immunoreactivities was noted when antisera to other anterior pituitary hormones were used. Biochemical and immunocytochemical examination of peptidylarginine deiminase during the pregnant period revealed its marked depletion on day 7 and its substantial restoration on day 14, showing apparently negative correlation with the known changes in the secretory activities of lactotrophs. This and the female-dominant occurrence of peptidylarginine deiminase in lactotrophs suggest significant involvement of the enzyme in the function of lactotrophs.
我们对大鼠垂体中的肽基精氨酸脱亚氨酶（EC 3.5.3.15）进行了免疫细胞化学研究。通过用布安-霍兰德升华液经心内短暂灌注固定雄性和雌性大鼠的垂体。用抗大鼠骨骼肌肽基精氨酸脱亚氨酶抗血清对雌性垂体获得的冰冻切片进行免疫过氧化物酶染色，在远侧部产生了许多细胞的特异性染色。相比之下，在雄性垂体远侧部仅发现少量免疫阳性细胞。雌性远侧部至少一些免疫阳性细胞也被抗大鼠催乳素抗血清染色。当使用针对其他垂体前叶激素的抗血清时，未观察到这种免疫反应性的巧合。对孕期肽基精氨酸脱亚氨酶的生化和免疫细胞化学检查显示，其在第7天显著减少，在第14天大量恢复，这显然与已知的催乳细胞分泌活动变化呈负相关。这以及肽基精氨酸脱亚氨酶在催乳细胞中以雌性为主的现象表明，该酶在催乳细胞功能中起重要作用。
