Emanuele M A, Tentler J, Emanuele N V, Reda D, Kirsteins L, Lawrence A M
Research and Medical Services, Veterans Administration Hospital, Hines, Illinois 60141.
Endocr Res. 1989;15(3):393-401. doi: 10.3109/07435808909042748.
The question of whether ethanol's (ETOH's) known suppressive effect on serum luteinizing hormone (LH) could be mediated directly at the anterior pituitary level was addressed by examining the effects of ETOH in vitro on release of LH from cultured male rat pituitary cells. The impact of added ethanol concentrations ranging from 50 to 400 mg% on LH release was examined in the basal state and after stimulation by gonadotropin-releasing hormone (GnRH) at a dose of 5 x 10(-10) M. While ETOH did not significantly suppress basal LH release, secretion stimulated with GnRH was noted to be attenuated with higher doses of ETOH (greater than or equal to 100 mg%) compared to stimulated control cells. It is concluded that ETOH exposure in vitro alters stimulated LH secretion by acting directly on pituitary gonadotropes.
通过检测乙醇（ETOH）在体外对培养的雄性大鼠垂体细胞释放促黄体生成素（LH）的影响，探讨了乙醇对血清促黄体生成素已知的抑制作用是否可直接在前脑垂体水平介导。在基础状态下以及在5×10（-10）M剂量的促性腺激素释放激素（GnRH）刺激后，检测了添加浓度范围为50至400mg%的乙醇对LH释放的影响。虽然乙醇没有显著抑制基础LH释放，但与受刺激的对照细胞相比，发现较高剂量的乙醇（大于或等于100mg%）会减弱GnRH刺激的分泌。得出的结论是，体外暴露于乙醇通过直接作用于垂体促性腺细胞来改变受刺激的LH分泌。
