Alfonso M, Durán R, Marcó J
Department of Fundamental Biology (Physiology), University of Vigo, Spain.
Endocr Res. 1993;19(2-3):175-86. doi: 10.3109/07435809309033023.
The effect of ethanol (EtOH) on LH and FSH release in vitro has been studied in order to determine whether the effect of alcohol observed in vivo could be mediated by a direct action at the level of the anterior pituitary. Pituitary glands were incubated for 4 hours in TC-199 medium with or with 100 mM ethanol. Basal gonadotrophin release was examined as well as secretion stimulated by luteinizing hormone releasing hormone (LHRH) in the presence or absence of cycloheximide. The total amount of LH and FSH released during the incubation period was calculated. Ethanol did not significantly alter basal gonadotrophin release or LHRH-stimulated FSH release. However, LH secretion induced by LHRH was decreased by EtOH when compared to the LHRH-stimulated group. Cycloheximide decreased the LHRH-stimulated release of LH and FSH but this effect was not altered by EtOH. The results show that EtOH alters the release but not the synthesis of gonadotrophins by a direct effect on the anterior pituitary gland.
为了确定体内观察到的酒精效应是否可能由垂体前叶水平的直接作用介导，研究了乙醇（EtOH）对体外促黄体生成素（LH）和促卵泡生成素（FSH）释放的影响。将垂体在含或不含100 mM乙醇的TC - 199培养基中孵育4小时。检测基础促性腺激素释放以及在存在或不存在放线菌酮的情况下，促黄体生成素释放激素（LHRH）刺激的分泌。计算孵育期间释放的LH和FSH总量。乙醇没有显著改变基础促性腺激素释放或LHRH刺激的FSH释放。然而，与LHRH刺激组相比，EtOH使LHRH诱导的LH分泌减少。放线菌酮降低了LHRH刺激的LH和FSH释放，但这种效应未被EtOH改变。结果表明，EtOH通过对垂体前叶的直接作用改变促性腺激素的释放而非合成。
