用于检测尿液样本中先天性人巨细胞病毒感染的实时定量聚合酶链反应检测方法的开发。
Development of a real-time quantitative polymerase chain reaction assay for the detection of congenital human cytomegalovirus infection in urine samples.
作者信息
Ligozzi Marco, Poggi Mara, Saletti Marilena, Gibellini Davide
机构信息
Microbiology and Virology Unit, Department of Pathology and Diagnostic, University of Verona, Strada Delle Grazie 8, 37134 Verona, Italy.
Microbiology and Virology Unit, Department of Pathology, Azienda Ospedaliera Universitaria Integrata of Verona, Piazzale Stefani 10, 35124 Verona, Italy.
出版信息
Mol Cell Probes. 2016 Feb;30(1):50-2. doi: 10.1016/j.mcp.2015.11.006. Epub 2015 Dec 8.
A TaqMan real-time quantitative PCR (qPCR), based on amplification of HCMV UL54 gene specific sequence, was developed and compared with shell vial viral culture assay, the gold standard technique for the diagnosis of congenital HCMV infection, using urine samples collected from 110 newborns. The results indicate that this qPCR is slightly more sensitive than shell vial assay suggesting that qPCR may be considered a useful alternative for diagnosing congenital HCMV infection.
基于人巨细胞病毒（HCMV）UL54基因特异性序列扩增的TaqMan实时定量聚合酶链反应（qPCR）技术被开发出来，并与空斑小室病毒培养检测法进行了比较。空斑小室病毒培养检测法是诊断先天性HCMV感染的金标准技术。研究使用了从110名新生儿收集的尿液样本。结果表明，这种qPCR技术比空斑小室检测法的敏感性略高，这表明qPCR技术可被视为诊断先天性HCMV感染的一种有用的替代方法。
Zotero 插件