Suzuki Y, Hashimoto K, Kato I, Nishioka K, Eto H, Nishiyama S, Kanzaki T
Department of Dermatology, Wayne State University, Detroit, MI 48201.
J Cutan Pathol. 1989 Apr;16(2):66-71. doi: 10.1111/j.1600-0560.1989.tb00013.x.
Several monoclonal antibodies (MAB) have been produced using an eccrine carcinoma cell line as an immunogen. One such MAB, SKH1, reacted with both the secretory portion and coiled duct of the eccrine and with the secretory portion of apocrine gland. SKH1, however, did not react with myoepithelial cells, intradermal ducts of both types of sweat gland, or with other components of normal axillary skin including the epidermis and follicular apparatus. The reaction was strongest if the specimen was fixed with 80% methanol, and moderate on non-fixed or acid-alcohol-fixed specimens. Only weak reaction was obtained on cold acetone-fixed specimens, and reaction was negative with formalin-fixed, paraffin-embedded tissues. SKH1 reacted positively with the cytoskeleton of the eccrine carcinoma cell line, Colo-16 and MCF-7. Applied to pathological skin specimens, SKH1 reacted with the tumor cells of clear cell hidradenoma, syringocystadenoma papilliferum, and extramammary Paget's disease. SKH1 also reacted with the tumor cells of metastatic adenocarcinomas arising from lung, breast and ovary. SKH1 did not react with the majority of tumor cells of eccrine poroma, but reacted with single-layered cells lining narrow ductal lumina. SKH1 did not react with epithelial cells lining cystic or ductal lumina of syringoma, but reacted moderately with the amorphous keratin-like substance filling the lumina. Immunoblot analysis revealed that SKH1 recognizes a 40 Kd sweat gland-associated antigen, and can be an aid to identifying tumors arising from sweat gland structures.
已经使用一种小汗腺癌细胞系作为免疫原制备了几种单克隆抗体（MAB）。其中一种MAB，即SKH1，与小汗腺的分泌部和蟠管以及大汗腺的分泌部都发生反应。然而，SKH1不与肌上皮细胞、两种汗腺的皮内导管或正常腋窝皮肤的其他成分（包括表皮和毛囊结构）发生反应。如果标本用80%甲醇固定，反应最强；在未固定或酸醇固定的标本上反应中等。在冷丙酮固定的标本上仅获得微弱反应，而在福尔马林固定、石蜡包埋的组织上反应为阴性。SKH1与小汗腺癌细胞系Colo-16和MCF-7的细胞骨架呈阳性反应。应用于病理皮肤标本时，SKH1与透明细胞汗腺瘤、乳头状汗腺囊腺瘤和乳腺外佩吉特病的肿瘤细胞发生反应。SKH1也与源自肺、乳腺和卵巢的转移性腺癌的肿瘤细胞发生反应。SKH1不与小汗腺汗孔瘤的大多数肿瘤细胞发生反应，但与狭窄管腔内衬的单层细胞发生反应。SKH1不与汗管瘤的囊性或管腔内衬上皮细胞发生反应，但与填充管腔的无定形角蛋白样物质发生中等强度反应。免疫印迹分析显示，SKH1识别一种40 Kd的汗腺相关抗原，可有助于识别源自汗腺结构的肿瘤。
