裂解而非内源性分泌的 RAGE 与急性缺血性脑卒中的结局相关。
Cleaved but not endogenous secretory RAGE is associated with outcome in acute ischemic stroke.
机构信息
From the Stroke Center and Department of Neurology (S.-C.T., S.-J.Y., L.-K.T., J.-S.J.), Department of Internal Medicine (W.-S.Y.), National Taiwan University Hospital; the Department of Neurology (C.-J.H.), Taipei Medical University Hospital and Shuang Ho Hospital; the Department of Neurology (L.-M.L.), Shin Kong Wu Ho-Su Memorial Hospital; the Department of Neurology (G.-S.P.), Tri-Service General Hospital; and the School of Public Health (H.-Y.C.), Taipei Medical University, Taipei, Taiwan.
出版信息
Neurology. 2016 Jan 19;86(3):270-6. doi: 10.1212/WNL.0000000000002287. Epub 2015 Dec 18.
OBJECTIVE
To investigate the expression patterns of 2 soluble isoforms of receptor for advanced glycation end-product (RAGE), including endogenous secretory RAGE (esRAGE) and cleaved RAGE (cRAGE), and their associations with outcome in acute ischemic stroke (IS).
METHODS
Acute IS patients (n = 106) and age- and sex-matched controls (n = 150) were recruited. Plasma levels of total soluble RAGE (sRAGE) and esRAGE in patients at <48 hours and 48-72 hours after IS and in controls were measured by ELISA. The level of cRAGE was calculated by subtracting the level of sRAGE from that of esRAGE. Poor outcome was defined as modified Rankin Scale score >2 at 3 months after stroke.
RESULTS
The plasma levels of cRAGE were significantly higher and correlated to those of esRAGE (p < 0.001). The plasma levels of esRAGE and cRAGE were both significantly higher in IS patients <48 hours and 48-72 hours after onset than in controls, but only level of cRAGE at <48 hours was independently associated with poor outcome after adjusting for clinical variables (odds ratio 2.44; 95% confidence interval 1.16-5.16; p = 0.019).
CONCLUSION
The plasma level of cRAGE at <48 hours after IS, rather than esRAGE, is a significant predictor of acute IS outcome.
目的
研究晚期糖基化终产物受体（RAGE）的 2 种可溶性同工型（包括内源性分泌型 RAGE（esRAGE）和裂解型 RAGE（cRAGE））的表达模式及其与急性缺血性脑卒中（IS）结局的关系。
方法
纳入 106 例急性 IS 患者和 150 例年龄和性别匹配的对照者。在 IS 发病后 <48 小时和 48-72 小时内，通过 ELISA 检测患者和对照者的总可溶性 RAGE（sRAGE）和 esRAGE 血浆水平。通过从 sRAGE 水平中减去 esRAGE 水平计算 cRAGE 水平。预后不良定义为卒中后 3 个月改良 Rankin 量表评分 >2 分。
结果
cRAGE 血浆水平显著升高，且与 esRAGE 水平呈正相关（p<0.001）。IS 患者在发病后 <48 小时和 48-72 小时时的 esRAGE 和 cRAGE 血浆水平均显著高于对照者，但只有 cRAGE 水平在发病后 <48 小时与临床变量调整后的不良预后独立相关（优势比 2.44；95%置信区间 1.16-5.16；p=0.019）。
结论
与 esRAGE 相比，IS 发病后 <48 小时的 cRAGE 血浆水平是急性 IS 结局的一个显著预测因子。
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