College of Life Science and Technology, Henan Institute of Science and Technology, Xinxiang 453003, PR China; College of Food Science and Technology, Nanjing Agricultural University, Nanjing 210095, PR China; Collaborative Innovation Center of Modern Biological Breeding, Xinxiang 453003, Henan Province, PR China.
College of Life Science and Technology, Henan Institute of Science and Technology, Xinxiang 453003, PR China; Collaborative Innovation Center of Modern Biological Breeding, Xinxiang 453003, Henan Province, PR China.
Carbohydr Polym. 2016 Feb 10;137:541-548. doi: 10.1016/j.carbpol.2015.11.010. Epub 2015 Nov 10.
Optimization of extraction, characterization and antioxidant activity of mycelial polysaccharides from Paecilomyces hepiali HN1 (PHMPs) were investigated. As results, the optimal parameters for extraction of PHMPs were obtained by a Box-Behnken design as follows: extraction temperature 92 °C, extracting time 190 min and ratio of water to material 43 mL/g. The analysis of monosaccharide composition by high performance liquid chromatography (HPLC) revealed that PHMPs was composed of mannose, ribose, rhamnose, glucuronic acid, galacturonic acid, glucose, galactose, xylose and arabinose in molar ratio of 46.07:0.59:2.25:1.29:1.42:18.82:26.17:1.13:2.26, respectively. Furthermore, it was demonstrated that PHMPs had a significant protective effect against oxidative stress induced by d-galactose in mice, as evident by higher activities of superoxide dismutase, catalase, glutathione peroxidase and level of total antioxidant capacity, as well as lower levels of malondialdehyde in serums and livers compared to the d-galactose-treated group. These results suggested that PHMPs could be explored as promising natural antioxidant.
优化了从白僵菌 HN1（PHMPs）中提取、表征和抗氧化活性的菌丝体多糖。结果表明，通过 Box-Behnken 设计得到了 PHMPs 的最佳提取参数为：提取温度 92°C，提取时间 190 min，料液比 43 mL/g。高效液相色谱（HPLC）分析单糖组成表明，PHMPs 由摩尔比为 46.07:0.59:2.25:1.29:1.42:18.82:26.17:1.13:2.26 的甘露糖、核糖、鼠李糖、葡萄糖醛酸、半乳糖醛酸、葡萄糖、半乳糖、木糖和阿拉伯糖组成。此外，研究还表明，PHMPs 对 d-半乳糖诱导的小鼠氧化应激具有显著的保护作用，表现在血清和肝脏中超氧化物歧化酶、过氧化氢酶、谷胱甘肽过氧化物酶活性和总抗氧化能力水平升高，丙二醛水平降低。这些结果表明，PHMPs 可以作为有前途的天然抗氧化剂进行探索。
