School of Basic Medicine, Qingdao University, Qingdao 266071, China.
Key Laboratory of Sustainable Development of Polar Fishery, Ministry of Agriculture and Rural Affairs, Yellow Sea Fisheries Research Institute, Chinese Academy of Fishery Sciences, Qingdao 266071, China.
Molecules. 2023 Jun 25;28(13):4984. doi: 10.3390/molecules28134984.
Cordyceps exopolysaccharide (CEP) has shown emerging potential in adjustment of gut microbiota and immune cell function. In this study, a water-soluble CEP with a molecular weight of 58.14 kDa was extracted from the fermentation broth of , an endophytic fungus of Cordyceps sinensis. Our results indicated that polysaccharide (PHP) showed significantly preventive potential on dextran sulfate sodium (DSS)-induced colitis in mice, which can prevent colon shortening, reduce intestinal epithelial cell (IEC) destruction, suppress inflammatory cell infiltration, and regulate the balance between regulatory T (Treg) cells and T helper type 17 (Th17) cells. Meanwhile, the disturbed gut microbiota was partially restored after PHP treatment. Further Pearson correlation coefficient analyses exhibited that the alteration of the gut microbiota was significantly related to adjustment of the IEC barrier and Treg/Th17 balance. In conclusion, all findings proposed that purified PHP has the potential to develop into a promising agent for colitis prevention and adjuvant therapy via maintaining intestinal homeostasis of gut microbiota and immune system.
蛹虫草胞外多糖（CEP）在调节肠道微生物群和免疫细胞功能方面显示出了新的潜力。在这项研究中，从蛹虫草内生真菌中提取了一种分子量为 58.14 kDa 的水溶性 CEP。我们的结果表明，多糖（PHP）对葡聚糖硫酸钠（DSS）诱导的结肠炎具有显著的预防潜力，可防止结肠缩短，减少肠上皮细胞（IEC）破坏，抑制炎症细胞浸润，并调节调节性 T（Treg）细胞和辅助性 T 细胞 17（Th17）细胞之间的平衡。同时，PHP 处理后部分恢复了失调的肠道微生物群。进一步的 Pearson 相关系数分析表明，肠道微生物群的改变与 IEC 屏障和 Treg/Th17 平衡的调节显著相关。总之，所有研究结果表明，纯化的 PHP 具有通过维持肠道微生物群和免疫系统的肠道内稳态来开发成一种有希望的结肠炎预防和辅助治疗剂的潜力。
