School of Pharmacy, Nanjing University of Chinese Medicine, Nanjing 210023, PR China; Jiangsu Key Laboratory of Research and Development in Marine Bio-resource Pharmaceutics, Nanjing University of Chinese Medicine, Nanjing 210023, PR China.
Center for Drug Safety Evaluation and Research, Nanjing University of Chinese Medicine, 210023, PR China.
Carbohydr Polym. 2016 Feb 10;137:709-718. doi: 10.1016/j.carbpol.2015.11.030. Epub 2015 Nov 19.
The polysaccharides from Mactra veneriformis (MVPS) were degraded by controlled mild acid hydrolysis to produce active oligosaccharides. MVPS can easily be hydrolyzed by H2SO4 or HCl. The hydrolyzing process was investigated in acid addition and reaction time by evaluating the producing content of reducing sugar. Hydrolysis with 1M HCl to MVPS can generate a time-depended behavior that is mild and controllable. HPLC analysis monitored the change of oligosaccharides composition in hydrolyzing. Total nine oligosaccharides are recognizable in the HPLC profile, and their content showed a regular transformation in hydrolysis. Those nine ingredients were identified as glucooligosaccharides with DP from 1 to 7 by MS analysis. Antioxidant activities of the typical hydrolyzates as well as MVPs were further tested in assays of DPPH and hydroxyl radicals scavenging, and reducing power. It was found that HCl hydrolyzate exhibited stronger antioxidant effects than MVPS and H2SO4 hydrolyzate due to its higher content of oligosaccharides.
来自文蛤（Mactra veneriformis）的多糖（MVPS）经控制温和酸水解降解，生成活性寡糖。MVPS 可通过 H2SO4 或 HCl 轻松水解。通过评估还原糖的生成含量来研究酸添加和反应时间对水解过程的影响。1M HCl 对 MVPS 的水解可产生温和且可控的时间依赖性行为。HPLC 分析监测了水解过程中寡糖组成的变化。在 HPLC 图谱中可识别出 9 种寡糖，其含量在水解过程中呈现出有规律的转化。通过 MS 分析，这些 9 种成分被鉴定为 DP 从 1 到 7 的葡萄糖寡糖。进一步测试了典型水解产物以及 MVPS 的 DPPH 和羟基自由基清除以及还原能力的抗氧化活性。结果发现，由于其更高的寡糖含量，HCl 水解产物比 MVPS 和 H2SO4 水解产物具有更强的抗氧化作用。
