未接触过任何处理的小鼠大脑中的喹啉酸免疫反应性。

Quinolinic acid-immunoreactivity in the naïve mouse brain.

作者信息

Lopez Yara Pujol, Kenis Gunter, Rutten Bart P F, Myint Aye M, Steinbusch Harry W M, van den Hove Daniel L A

机构信息

School for Mental Health and Neuroscience (MHeNS), Department of Psychiatry and Neuropsychology, Maastricht University Medical Centre+, Maastricht, The Netherlands.

出版信息

J Chem Neuroanat. 2016 Jan;71:6-12. doi: 10.1016/j.jchemneu.2015.10.010. Epub 2015 Dec 12.

DOI:10.1016/j.jchemneu.2015.10.010

PMID:26686288

Abstract

Quinolinic acid (QUIN) has been suggested to be involved in infections, inflammatory neurological disorders and in the development of psychiatric disorders. In this view, several studies have been performed to investigate QUIN localization in the brain and its neurotoxic effects. However, evidence is lacking regarding QUIN in healthy, control conditions. The aim of this study was to investigate the region-specific distribution and pattern of QUIN expression in the naïve mouse brain. In addition, possible sex differences in QUIN-immunoreactivity and its link with affect-related behavioural observations were assessed. For this purpose, naïve mice were subjected to the forced swim test (FST) and 20 min open field (OF) testing to measure affect-related behaviour. Afterwards, brains were assessed for QUIN-immunoreactivity. QUIN-immunoreactivity was particularly observed in the cingulate cortex (CC), highlighting clearly delineated cells, and the thalamic reticular nucleus (TRN), showing a more diffuse staining pattern. Subsequently, QUIN-positive cells in the CC were counted, while QUIN-immunoreactivity in the TRN was examined using gray value measurements. No significant differences between sexes were observed for the number of QUIN-positive cells in the CC, neither in levels of QUIN-immunoreactivity in the TRN. A direct correlation was found between QUIN-positive cells in the CC and QUIN-immunoreactivity in the TRN. Moreover, in male mice, a very strong correlation (rsp=.943; p<.01) between QUIN-immunoreactivity at the level of the TRN and motor activity in the OF was observed. Thus, our results suggest that QUIN - detected in the CC and the TRN - may play a role in regulating motor activity in normal conditions.

摘要

喹啉酸（QUIN）被认为与感染、炎症性神经疾病以及精神疾病的发展有关。基于此观点，已经开展了多项研究来调查QUIN在大脑中的定位及其神经毒性作用。然而，在健康对照条件下关于QUIN的证据尚缺。本研究的目的是调查未接触过任何处理的小鼠大脑中QUIN表达的区域特异性分布和模式。此外，还评估了QUIN免疫反应性可能存在的性别差异及其与情感相关行为观察结果的联系。为此，对未接触过任何处理的小鼠进行强迫游泳试验（FST）和20分钟旷场试验（OF）以测量情感相关行为。之后，对大脑进行QUIN免疫反应性评估。在扣带回皮质（CC）特别观察到QUIN免疫反应性，突出显示出清晰界定的细胞，以及丘脑网状核（TRN），呈现出更弥散的染色模式。随后，对CC中的QUIN阳性细胞进行计数，同时使用灰度值测量来检查TRN中的QUIN免疫反应性。在CC中，QUIN阳性细胞的数量在两性之间未观察到显著差异，TRN中的QUIN免疫反应性水平也未观察到显著差异。发现CC中的QUIN阳性细胞与TRN中的QUIN免疫反应性之间存在直接相关性。此外，在雄性小鼠中，观察到TRN水平的QUIN免疫反应性与OF中的运动活动之间存在非常强的相关性（rsp = 0.943；p < 0.01）。因此，我们的结果表明，在CC和TRN中检测到的QUIN可能在正常条件下调节运动活动中发挥作用。