结核病、肺癌和慢性阻塞性肺疾病中的DNA损伤与细胞异常
DNA damage and cellular abnormalities in tuberculosis, lung cancer and chronic obstructive pulmonary disease.
作者信息
da Silva Andréa Lúcia Gonçalves, Bresciani Maribel Josimara, Karnopp Thaís Evelyn, Weber Augusto Ferreira, Ellwanger Joel Henrique, Henriques João Antonio Pêgas, Valim Andréia Rosane de Moura, Possuelo Lia Gonçalves
机构信息
Department of Health and Physical Education, University of Santa Cruz do Sul - UNISC, Avenida Independência, 2293, Bairro Universitário, Santa Cruz do Sul, RS CEP 96815-900 Brazil.
Department of Biology and Pharmacy, University of Santa Cruz do Sul - UNISC, Santa Cruz do Sul, RS Brazil.
出版信息
Multidiscip Respir Med. 2015 Dec 19;10:38. doi: 10.1186/s40248-015-0034-z. eCollection 2015.
BACKGROUND
Tuberculosis (TB), Lung Cancer (LC) and Chronic Obstructive Pulmonary Diseases (COPD) affect millions of individuals worldwide. Monitoring of DNA damage in pathological situations has been investigated because it can add a new dimension to clinical expression and may represent a potential target for therapeutic intervention. The aim of this study was to evaluate DNA damage and the frequency of cellular abnormalities in TB, LC and COPD patients by comparing them to healthy subjects.
METHODS
The detection of DNA damage by a buccal micronucleus cytome assay was investigated in patients with COPD (n = 28), LC (n = 18) and TB (n = 22) and compared to control individuals (n = 17).
RESULTS
The COPD group had a higher frequency of apoptotic cells compared to TB and LC group. The TB group showed a higher frequency of DNA damage, defect in cytokinesis, apoptotic and necrotic cells. Patients with LC had low frequency of chromosomal aberrations than TB and COPD patients.
CONCLUSION
COPD patients showed cellular abnormalities that corresponded to cell death by apoptosis and necrosis, while patients with TB presented defects in cytokinesis and dysfunctions in DNA repair that resulted in the formation of micronucleus (MN) besides apoptotic and necrotic cells. Patients with COPD, TB and LC had a low frequency of permanent DNA damage.
背景
结核病（TB）、肺癌（LC）和慢性阻塞性肺疾病（COPD）影响着全球数百万人。对病理情况下DNA损伤的监测已得到研究，因为它可为临床表型增添新的维度，并且可能代表治疗干预的潜在靶点。本研究的目的是通过将结核病、肺癌和慢性阻塞性肺疾病患者与健康受试者进行比较，评估他们的DNA损伤和细胞异常频率。
方法
采用口腔微核细胞分析法对慢性阻塞性肺疾病患者（n = 28）、肺癌患者（n = 18）和结核病患者（n = 22）进行DNA损伤检测，并与对照组个体（n = 17）进行比较。
结果
与结核病和肺癌组相比，慢性阻塞性肺疾病组的凋亡细胞频率更高。结核病组显示出更高的DNA损伤频率、胞质分裂缺陷、凋亡和坏死细胞频率。肺癌患者的染色体畸变频率低于结核病和慢性阻塞性肺疾病患者。
结论
慢性阻塞性肺疾病患者表现出与凋亡和坏死导致的细胞死亡相对应的细胞异常，而结核病患者则表现出胞质分裂缺陷和DNA修复功能障碍，除凋亡和坏死细胞外还导致微核（MN）形成。慢性阻塞性肺疾病、结核病和肺癌患者的永久性DNA损伤频率较低。
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