Brody H, Carbon J
Department of Biological Sciences, University of California, Santa Barbara 93106.
Proc Natl Acad Sci U S A. 1989 Aug;86(16):6260-3. doi: 10.1073/pnas.86.16.6260.
An electrophoretic karyotype of Aspergillus nidulans has been obtained using contour-clamped homogeneous electric field gel electrophoresis. Six chromosomal bands were separated, with two of the bands migrating as doublets. Using the Schizosaccharomyces pombe and Saccharomyces cerevisiae chromosomes as size standards, we estimate the sizes of the chromosomes to be between 2.9 and 5.0 megabase pairs (mb) with a total genome size of approximately 31 mb. Four of the eight genetic linkage groups were assigned to chromosomal bands by hybridization of contour-clamped homogeneous electric field gel blots with various radiolabeled probes each specific to a particular linkage group. Contour-clamped homogeneous electric field gel analysis of reciprocal translocation strains gave chromosomal assignments for the four remaining linkage groups. In order of decreasing size, the A. nidulans chromosomes are: VIII (5.0 mb), VII (4.5 mb), II (4.2 mb), I and V (3.8 mb), III and VI (3.5 mb), and IV (2.9 mb).
利用轮廓夹钳均匀电场凝胶电泳技术获得了构巢曲霉的电泳核型。分离出了六条染色体带，其中两条带以双条带形式迁移。以粟酒裂殖酵母和酿酒酵母的染色体作为大小标准，我们估计这些染色体的大小在2.9至5.0兆碱基对（mb）之间，基因组总大小约为31 mb。通过将轮廓夹钳均匀电场凝胶印迹与各种分别针对特定连锁群的放射性标记探针杂交，八个遗传连锁群中的四个被定位到染色体带上。对相互易位菌株进行轮廓夹钳均匀电场凝胶分析，确定了其余四个连锁群的染色体定位。按照大小递减顺序，构巢曲霉的染色体依次为：第八条（5.0 mb）、第七条（4.5 mb）、第二条（4.2 mb）、第一条和第五条（3.8 mb）、第三条和第六条（3.5 mb）以及第四条（2.9 mb）。
