• 文献检索
  • 文档翻译
  • 深度研究
  • 学术资讯
  • Suppr Zotero 插件Zotero 插件
  • 邀请有礼
  • 套餐&价格
  • 历史记录
应用&插件
Suppr Zotero 插件Zotero 插件浏览器插件Mac 客户端Windows 客户端微信小程序
定价
高级版会员购买积分包购买API积分包
服务
文献检索文档翻译深度研究API 文档MCP 服务
关于我们
关于 Suppr公司介绍联系我们用户协议隐私条款
关注我们

Suppr 超能文献

核心技术专利：CN118964589B侵权必究
粤ICP备2023148730 号-1Suppr @ 2026

文献检索

告别复杂PubMed语法，用中文像聊天一样搜索，搜遍4000万医学文献。AI智能推荐，让科研检索更轻松。

立即免费搜索

文件翻译

保留排版，准确专业，支持PDF/Word/PPT等文件格式，支持 12+语言互译。

免费翻译文档

深度研究

AI帮你快速写综述，25分钟生成高质量综述，智能提取关键信息，辅助科研写作。

立即免费体验

酵母的电泳核型。

An electrophoretic karyotype for yeast.

作者信息

Carle G F, Olson M V

出版信息

Proc Natl Acad Sci U S A. 1985 Jun;82(11):3756-60. doi: 10.1073/pnas.82.11.3756.

DOI:10.1073/pnas.82.11.3756
PMID:3889913
原文链接:https://pmc.ncbi.nlm.nih.gov/articles/PMC397866/
Abstract

The chromosomal DNA molecules of a standard laboratory strain of Saccharomyces cerevisiae have been separated into 12 well-resolved bands by orthogonal-field-alternation gel electrophoresis. DNA X DNA hybridization probes derived from cloned genes have been used to correlate this banding pattern with yeast's genetically defined chromosomes. The 12 bands are shown to represent 9 singlets and 3 comigrating doublets, thereby accounting for 15 chromosomes that were identified as I-XI and XIII-XVI. Because the three comigrating doublets could be readily resolved in certain laboratory yeast strains that contain chromosome-length polymorphisms relative to our standard strain, all 15 of these chromosomes could be displayed as a single band in at least one of four strains that were studied. A 16th chromosome (number XII), which is known to contain the genes for rRNA, does not reproducibly enter the gels. By making use of the band identifications, the previously unmapped fragment F8 was assigned to chromosome XIII. With the possible exception of chromosomes that differ greatly in size or electrophoretic behavior from all the known chromosomes, the results appear to define a complete "electrophoretic karyotype" for yeast.

摘要

通过正交交变电场凝胶电泳，酿酒酵母标准实验室菌株的染色体DNA分子已被分离成12条分辨率良好的条带。源自克隆基因的DNA×DNA杂交探针已被用于将这种条带模式与酵母的遗传学定义染色体相关联。这12条条带显示代表9个单条带和3个共迁移的双条带，从而对应于鉴定为I - XI和XIII - XVI的15条染色体。由于在相对于我们的标准菌株含有染色体长度多态性的某些实验室酵母菌株中，这三个共迁移的双条带能够很容易地分辨出来，所以在研究的四个菌株中的至少一个中，所有这15条染色体都可以显示为一条单带。已知含有rRNA基因的第16号染色体（编号XII）不能重复性地进入凝胶。通过利用条带鉴定，先前未定位的片段F8被定位到染色体XIII上。除了那些在大小或电泳行为上与所有已知染色体有很大差异的染色体外，这些结果似乎定义了酵母完整的“电泳核型”。

https://cdn.ncbi.nlm.nih.gov/pmc/blobs/d74d/397866/ba965367df39/pnas00351-0235-c.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/d74d/397866/ac3be3a20c34/pnas00351-0233-a.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/d74d/397866/3a0cdef8f960/pnas00351-0234-a.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/d74d/397866/662d0429a57f/pnas00351-0234-b.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/d74d/397866/58fd25b67681/pnas00351-0234-c.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/d74d/397866/0c06ec7ddc20/pnas00351-0235-a.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/d74d/397866/835f27315f87/pnas00351-0235-b.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/d74d/397866/ba965367df39/pnas00351-0235-c.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/d74d/397866/ac3be3a20c34/pnas00351-0233-a.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/d74d/397866/3a0cdef8f960/pnas00351-0234-a.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/d74d/397866/662d0429a57f/pnas00351-0234-b.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/d74d/397866/58fd25b67681/pnas00351-0234-c.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/d74d/397866/0c06ec7ddc20/pnas00351-0235-a.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/d74d/397866/835f27315f87/pnas00351-0235-b.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/d74d/397866/ba965367df39/pnas00351-0235-c.jpg

相似文献

1
An electrophoretic karyotype for yeast.酵母的电泳核型。
Proc Natl Acad Sci U S A. 1985 Jun;82(11):3756-60. doi: 10.1073/pnas.82.11.3756.
2
Comparison of the separation of Candida albicans chromosome-sized DNA by pulsed-field gel electrophoresis techniques.利用脉冲场凝胶电泳技术分离白色念珠菌染色体大小DNA的比较。
Nucleic Acids Res. 1989 May 25;17(10):3783-93. doi: 10.1093/nar/17.10.3783.
3
The electrophoretic karyotype of two strains of Candida albicans by transverse alternate field electrophoresis reveals higher number of chromosomes ranging from 1 to 3.5 Mb.通过横向交变电场电泳对两株白色念珠菌进行的电泳核型分析显示，其染色体数量较多，大小在1至3.5兆碱基之间。
Yeast. 1993 Nov;9(11):1213-8. doi: 10.1002/yea.320091108.
4
Separation of chromosomal DNA molecules from yeast by orthogonal-field-alternation gel electrophoresis.通过正交交变电场凝胶电泳从酵母中分离染色体DNA分子。
Nucleic Acids Res. 1984 Jul 25;12(14):5647-64. doi: 10.1093/nar/12.14.5647.
5
[Chromosome polymorphism in the yeast Saccharomyces].[酵母酿酒酵母中的染色体多态性]
Genetika. 1990 Dec;26(12):2135-46.
6
Separation of chromosomes of Cryptococcus neoformans by pulsed field gel electrophoresis.通过脉冲场凝胶电泳分离新型隐球菌的染色体
Infect Immun. 1989 Sep;57(9):2624-7. doi: 10.1128/iai.57.9.2624-2627.1989.
7
An electrophoretic karyotype of the cultivated mushroom--Agaricus bisporus.栽培蘑菇双孢蘑菇的电泳核型。
Curr Genet. 1993 Dec;24(6):496-9. doi: 10.1007/BF00351712.
8
Variation in the electrophoretic karyotype analysed by the assignment of DNA probes in Candida albicans.通过在白色念珠菌中分配DNA探针分析电泳核型的变异。
J Gen Microbiol. 1990 Dec;136(12):2433-42. doi: 10.1099/00221287-136-12-2433.
9
Isolation of yeast artificial chromosomes free of endogenous yeast chromosomes: construction of alternate hosts with defined karyotypic alterations.不含内源性酵母染色体的酵母人工染色体的分离：构建具有特定核型改变的替代宿主。
Proc Natl Acad Sci U S A. 1995 Dec 5;92(25):11706-10. doi: 10.1073/pnas.92.25.11706.
10
DNA translocations contribute to chromosome length polymorphisms in Candida albicans.DNA易位导致白色念珠菌的染色体长度多态性。
Curr Genet. 1992 Aug;22(2):93-100. doi: 10.1007/BF00351467.

引用本文的文献

1
The TOG protein Stu2 is regulated by acetylation.TOG 蛋白 Stu2 通过乙酰化进行调节。
PLoS Genet. 2022 Sep 9;18(9):e1010358. doi: 10.1371/journal.pgen.1010358. eCollection 2022 Sep.
2
SRAS-net: Low-resolution chromosome image classification based on deep learning.SRAS-net：基于深度学习的低分辨率染色体图像分类。
IET Syst Biol. 2022 May;16(3-4):85-97. doi: 10.1049/syb2.12042. Epub 2022 Apr 4.
3
Studying DNA Double-Strand Break Repair: An Ever-Growing Toolbox.研究DNA双链断裂修复：一个不断扩充的工具箱。

本文引用的文献

1
Genetic Mapping in Saccharomyces IV. Mapping of Temperature-Sensitive Genes and Use of Disomic Strains in Localizing Genes.酵母的遗传图谱 IV. 温度敏感基因的图谱绘制以及在定位基因中的二倍体菌株的使用。
Genetics. 1973 May;74(1):33-54. doi: 10.1093/genetics/74.1.33.
2
Replication and recombination functions associated with the yeast plasmid, 2 mu circle.与酵母质粒2μm环相关的复制和重组功能。
Cell. 1980 Sep;21(2):501-8. doi: 10.1016/0092-8674(80)90487-0.
3
Genetic map of Saccharomyces cerevisiae.酿酒酵母的遗传图谱。
Front Mol Biosci. 2020 Feb 21;7:24. doi: 10.3389/fmolb.2020.00024. eCollection 2020.
4
Streamlined preparation of genomic DNA in agarose plugs for pulsed-field gel electrophoresis.用于脉冲场凝胶电泳的琼脂糖凝胶块中基因组DNA的简化制备方法。
J Biol Methods. 2018 Mar 9;5(1):e86. doi: 10.14440/jbm.2018.218. eCollection 2018.
5
Whole Genome Sequencing, Assembly and Phenotypic Profiling for the New Budding Yeast Species .新型出芽酵母物种的全基因组测序、组装及表型分析
G3 (Bethesda). 2018 Aug 30;8(9):2967-2977. doi: 10.1534/g3.118.200476.
6
Evaluating fermentation characteristics of Kazachstania spp. and their potential influence on wine quality.评估哈萨克斯坦酵母属的发酵特性及其对葡萄酒品质的潜在影响。
World J Microbiol Biotechnol. 2017 Jul;33(7):129. doi: 10.1007/s11274-017-2299-1. Epub 2017 Jun 5.
7
The genetics of a putative social trait in natural populations of yeast.酵母自然种群中一种假定社会性状的遗传学。
Mol Ecol. 2014 Oct;23(20):5061-71. doi: 10.1111/mec.12904. Epub 2014 Oct 4.
8
Preparation of megabase-sized tomato DNA and separation of large restriction fragments by field inversion gel electrophoresis (FIGE).大片段番茄 DNA 的制备及场反转凝胶电泳（FIGE）分离大片段限制酶切片段。
Plant Mol Biol. 1989 Mar;12(3):341-52. doi: 10.1007/BF00043211.
9
Analysis of DNA from a Beta procumbens chromosome fragment in sugar beet carrying a gene for nematode resistance.分析携带抗线虫基因的甜菜β procumbens 染色体片段中的 DNA。
Theor Appl Genet. 1990 May;79(5):663-72. doi: 10.1007/BF00226881.
10
Preparation of high molecular weight plant DNA and its use for artificial chromosome construction.高分子量植物 DNA 的制备及其在人工染色体构建中的应用。
Plant Cell Rep. 1991 Sep;10(6-7):315-20. doi: 10.1007/BF00193149.
Microbiol Rev. 1980 Dec;44(4):519-71. doi: 10.1128/mr.44.4.519-571.1980.
4
Quantitative karyotyping of human chromosomes by dual beam flow cytometry.通过双光束流式细胞术对人类染色体进行定量核型分析。
Proc Natl Acad Sci U S A. 1982 Dec;79(24):7876-80. doi: 10.1073/pnas.79.24.7876.
5
Correspondence of yeast UAA suppressors to cloned tRNASerUCA genes.酵母UAA抑制子与克隆的tRNASerUCA基因的对应关系。
J Mol Biol. 1981 Aug 15;150(3):375-87. doi: 10.1016/0022-2836(81)90553-2.
6
Insertion of a repetitive element at the same position in the 5'-flanking regions of two dissimilar yeast tRNA genes.在两个不同的酵母tRNA基因的5'侧翼区域的相同位置插入一个重复元件。
Proc Natl Acad Sci U S A. 1982 Dec;79(24):7674-8. doi: 10.1073/pnas.79.24.7674.
7
Chromosome rearrangements in Trypanosoma brucei.布氏锥虫中的染色体重排
Cell. 1984 Nov;39(1):213-21. doi: 10.1016/0092-8674(84)90207-1.
8
Rearrangements of highly polymorphic regions near telomeres of Saccharomyces cerevisiae.酿酒酵母端粒附近高度多态性区域的重排
Mol Cell Biol. 1984 Nov;4(11):2509-17. doi: 10.1128/mcb.4.11.2509-2517.1984.
9
Separation of chromosomal DNA molecules from yeast by orthogonal-field-alternation gel electrophoresis.通过正交交变电场凝胶电泳从酵母中分离染色体DNA分子。
Nucleic Acids Res. 1984 Jul 25;12(14):5647-64. doi: 10.1093/nar/12.14.5647.
10
Separation of yeast chromosome-sized DNAs by pulsed field gradient gel electrophoresis.通过脉冲场梯度凝胶电泳分离酵母染色体大小的DNA。
Cell. 1984 May;37(1):67-75. doi: 10.1016/0092-8674(84)90301-5.