Smith Carlas, Lari Azra, Derrer Carina Patrizia, Ouwehand Anette, Rossouw Ammeret, Huisman Maximiliaan, Dange Thomas, Hopman Mark, Joseph Aviva, Zenklusen Daniel, Weis Karsten, Grunwald David, Montpetit Ben
RNA Therapeutics Institute, University of Massachusetts Medical School, Worcester, MA 01605 Department Biochemistry and Molecular Pharmacology, University of Massachusetts Medical School, Worcester, MA 01605.
Department of Cell Biology, University of Alberta, T6G 2H7 Edmonton, Alberta, Canada.
J Cell Biol. 2015 Dec 21;211(6):1121-30. doi: 10.1083/jcb.201503135.
Many messenger RNA export proteins have been identified; yet the spatial and temporal activities of these proteins and how they determine directionality of messenger ribonucleoprotein (mRNP) complex export from the nucleus remain largely undefined. Here, the bacteriophage PP7 RNA-labeling system was used in Saccharomyces cerevisiae to follow single-particle mRNP export events with high spatial precision and temporal resolution. These data reveal that mRNP export, consisting of nuclear docking, transport, and cytoplasmic release from a nuclear pore complex (NPC), is fast (∼ 200 ms) and that upon arrival in the cytoplasm, mRNPs are frequently confined near the nuclear envelope. Mex67p functions as the principal mRNP export receptor in budding yeast. In a mex67-5 mutant, delayed cytoplasmic release from NPCs and retrograde transport of mRNPs was observed. This proves an essential role for Mex67p in cytoplasmic mRNP release and directionality of transport.
许多信使核糖核酸（mRNA）输出蛋白已被鉴定出来；然而，这些蛋白的时空活性以及它们如何决定信使核糖核蛋白（mRNP）复合物从细胞核输出的方向性，在很大程度上仍不明确。在这里，噬菌体PP7 RNA标记系统被用于酿酒酵母中，以高空间精度和时间分辨率追踪单颗粒mRNP输出事件。这些数据表明，mRNP输出包括从核孔复合体（NPC）进行核对接、运输和细胞质释放，速度很快（约200毫秒），并且在到达细胞质后，mRNP经常被限制在核膜附近。Mex67p在出芽酵母中作为主要的mRNP输出受体发挥作用。在mex67 - 5突变体中，观察到从NPCs的细胞质释放延迟以及mRNP的逆行运输。这证明了Mex67p在细胞质mRNP释放和运输方向性中起着至关重要的作用。
Zotero 插件
