核mRNA输出需要Mex67p和Mtr2p在核孔处形成复合物。
Nuclear mRNA export requires complex formation between Mex67p and Mtr2p at the nuclear pores.
作者信息
Santos-Rosa H, Moreno H, Simos G, Segref A, Fahrenkrog B, Panté N, Hurt E
机构信息
Biochemie-Zentrum Heidelberg, D-69120 Heidelberg, Germany.
出版信息
Mol Cell Biol. 1998 Nov;18(11):6826-38. doi: 10.1128/MCB.18.11.6826.
Abstract
We have identified between Mex67p and Mtr2p a complex which is essential for mRNA export. This complex, either isolated from yeast or assembled in Escherichia coli, can bind in vitro to RNA through Mex67p. In vivo, Mex67p requires Mtr2p for association with the nuclear pores, which can be abolished by mutating either MEX67 or MTR2. In all cases, detachment of Mex67p from the pores into the cytoplasm correlates with a strong inhibition of mRNA export. At the nuclear pores, Nup85p represents one of the targets with which the Mex67p-Mtr2p complex interacts. Thus, Mex67p and Mtr2p constitute a novel mRNA export complex which can bind to RNA via Mex67p and which interacts with nuclear pores via Mtr2p.
摘要
我们在Mex67p和Mtr2p之间鉴定出一种对mRNA输出至关重要的复合体。这种复合体，无论是从酵母中分离出来的还是在大肠杆菌中组装的，都能在体外通过Mex67p与RNA结合。在体内，Mex67p需要Mtr2p才能与核孔结合，而通过突变MEX67或MTR2都可以消除这种结合。在所有情况下，Mex67p从核孔脱离进入细胞质都与mRNA输出的强烈抑制相关。在核孔处，Nup85p是Mex67p-Mtr2p复合体相互作用的靶标之一。因此，Mex67p和Mtr2p构成了一种新型的mRNA输出复合体，它可以通过Mex67p与RNA结合，并通过Mtr2p与核孔相互作用。
相似文献
1
Nuclear mRNA export requires complex formation between Mex67p and Mtr2p at the nuclear pores.核mRNA输出需要Mex67p和Mtr2p在核孔处形成复合物。
Mol Cell Biol. 1998 Nov;18(11):6826-38. doi: 10.1128/MCB.18.11.6826.
2
Binding of the Mex67p/Mtr2p heterodimer to FXFG, GLFG, and FG repeat nucleoporins is essential for nuclear mRNA export.Mex67p/Mtr2p异二聚体与FXFG、GLFG和FG重复核孔蛋白的结合对于核mRNA输出至关重要。
J Cell Biol. 2000 Aug 21;150(4):695-706. doi: 10.1083/jcb.150.4.695.
3
Mex67p, a novel factor for nuclear mRNA export, binds to both poly(A)+ RNA and nuclear pores.Mex67p是一种新型的核mRNA输出因子，它既能与聚腺苷酸（poly(A)）+ RNA结合，也能与核孔结合。
EMBO J. 1997 Jun 2;16(11):3256-71. doi: 10.1093/emboj/16.11.3256.
4
Yra1p, a conserved nuclear RNA-binding protein, interacts directly with Mex67p and is required for mRNA export.Yra1p是一种保守的核RNA结合蛋白，它直接与Mex67p相互作用，是mRNA输出所必需的。
EMBO J. 2000 Feb 1;19(3):410-20. doi: 10.1093/emboj/19.3.410.
5
The GLFG regions of Nup116p and Nup100p serve as binding sites for both Kap95p and Mex67p at the nuclear pore complex.Nup116p和Nup100p的GLFG区域在核孔复合体中作为Kap95p和Mex67p的结合位点。
J Biol Chem. 2001 Mar 2;276(9):6445-52. doi: 10.1074/jbc.M008311200. Epub 2000 Dec 4.
6
The Mex67p-mediated nuclear mRNA export pathway is conserved from yeast to human.Mex67p介导的核mRNA输出途径在从酵母到人类的生物中是保守的。
EMBO J. 1999 May 4;18(9):2593-609. doi: 10.1093/emboj/18.9.2593.
7
Splicing factor Sub2p is required for nuclear mRNA export through its interaction with Yra1p.剪接因子Sub2p通过与Yra1p相互作用，对核mRNA输出是必需的。
Nature. 2001 Oct 11;413(6856):648-52. doi: 10.1038/35098113.
8
The mRNA export machinery requires the novel Sac3p-Thp1p complex to dock at the nucleoplasmic entrance of the nuclear pores.信使核糖核酸（mRNA）输出机制需要新型的Sac3p-Thp1p复合物停靠在核孔的核质入口处。
EMBO J. 2002 Nov 1;21(21):5843-52. doi: 10.1093/emboj/cdf590.
9
Rat8p/Dbp5p is a shuttling transport factor that interacts with Rat7p/Nup159p and Gle1p and suppresses the mRNA export defect of xpo1-1 cells.Rat8p/Dbp5p是一种穿梭运输因子，它与Rat7p/Nup159p和Gle1p相互作用，并抑制xpo1-1细胞的mRNA输出缺陷。
EMBO J. 1999 Oct 15;18(20):5778-88. doi: 10.1093/emboj/18.20.5778.
10
The yeast hnRNP-Like proteins Yra1p and Yra2p participate in mRNA export through interaction with Mex67p.酵母类异质性核糖核蛋白Yra1p和Yra2p通过与Mex67p相互作用参与mRNA输出。
Mol Cell Biol. 2001 Jul;21(13):4219-32. doi: 10.1128/MCB.21.13.4219-4232.2001.
引用本文的文献
1
Unraveling gene expression: a beginner's guide from chromatin modifications to mRNA export in .解析基因表达：从染色质修饰到mRNA输出的初学者指南
Nucleus. 2025 Dec;16(1):2516909. doi: 10.1080/19491034.2025.2516909. Epub 2025 Jun 13.
2
The SARS-CoV-2 ORF6 protein inhibits nuclear export of mRNA and spliceosomal U snRNA.SARS-CoV-2 的 ORF6 蛋白抑制 mRNA 的核输出和剪接体 U snRNA。
PLoS One. 2024 Oct 31;19(10):e0312098. doi: 10.1371/journal.pone.0312098. eCollection 2024.
3
The RNA helicase DDX39 contributes to the nuclear export of spliceosomal U snRNA by loading of PHAX onto RNA.RNA 解旋酶 DDX39 通过将 PHAX 加载到 RNA 上来促进剪接体 U snRNA 的核输出。
Nucleic Acids Res. 2024 Sep 23;52(17):10668-10682. doi: 10.1093/nar/gkae622.
4
Single-molecule quantitation of RNA-binding protein occupancy and stoichiometry defines a role for Yra1 (Aly/REF) in nuclear mRNP organization.单分子定量分析 RNA 结合蛋白占有率和化学计量比，定义了 Yra1（Aly/REF）在核 mRNP 组织中的作用。
Cell Rep. 2023 Nov 28;42(11):113415. doi: 10.1016/j.celrep.2023.113415. Epub 2023 Nov 14.
5
Nuclear mRNA metabolism drives selective basket assembly on a subset of nuclear pore complexes in budding yeast.核 mRNA 代谢驱动了芽殖酵母中一部分核孔复合物上的选择性篮状复合物的组装。
Mol Cell. 2022 Oct 20;82(20):3856-3871.e6. doi: 10.1016/j.molcel.2022.09.019. Epub 2022 Oct 10.
6
Sending the message: specialized RNA export mechanisms in trypanosomes.传递信息：锥虫中特化的 RNA 输出机制。
Trends Parasitol. 2022 Oct;38(10):854-867. doi: 10.1016/j.pt.2022.07.008. Epub 2022 Aug 24.
7
Eukaryotic Ribosome assembly and Nucleocytoplasmic Transport.真核生物核糖体的组装和核质转运。
Methods Mol Biol. 2022;2533:99-126. doi: 10.1007/978-1-0716-2501-9_7.
8
The C-terminal tail of ribosomal protein Rps15 is engaged in cytoplasmic pre-40S maturation.核糖体蛋白Rps15的C末端尾巴参与细胞质中40S前体的成熟过程。
RNA Biol. 2022;19(1):560-574. doi: 10.1080/15476286.2022.2064073. Epub 2021 Dec 31.
9
The Mammalian Ecdysoneless Protein Interacts with RNA Helicase DDX39A To Regulate Nuclear mRNA Export.哺乳动物蜕皮甾酮缺乏蛋白与 RNA 解旋酶 DDX39A 相互作用，以调节核 mRNA 输出。
Mol Cell Biol. 2021 Jun 23;41(7):e0010321. doi: 10.1128/MCB.00103-21.
10
Nuclear mRNA maturation and mRNA export control: from trypanosomes to opisthokonts.核 mRNA 成熟和 mRNA 输出控制：从原生动物到后口动物。
Parasitology. 2021 Sep;148(10):1196-1218. doi: 10.1017/S0031182021000068. Epub 2021 Jan 19.
本文引用的文献
1
Use of the A. victoria green fluorescent protein to study protein dynamics in vivo.利用维多利亚多管水母绿色荧光蛋白研究体内蛋白质动力学。
Curr Protoc Mol Biol. 2001 May;Chapter 9:Unit9.7C. doi: 10.1002/0471142727.mb0907cs34.
2
TAP, the human homolog of Mex67p, mediates CTE-dependent RNA export from the nucleus.TAP是Mex67p的人类同源物，介导依赖CTE的RNA从细胞核输出。
Mol Cell. 1998 Apr;1(5):649-59. doi: 10.1016/s1097-2765(00)80065-9.
3
Mtr10p functions as a nuclear import receptor for the mRNA-binding protein Npl3p.Mtr10p作为mRNA结合蛋白Npl3p的核输入受体发挥作用。
EMBO J. 1998 Apr 15;17(8):2196-207. doi: 10.1093/emboj/17.8.2196.
4
A distinct and parallel pathway for the nuclear import of an mRNA-binding protein.一条用于mRNA结合蛋白核输入的独特且平行的途径。
J Cell Biol. 1997 Dec 29;139(7):1645-53. doi: 10.1083/jcb.139.7.1645.
5
CRM1 is responsible for intracellular transport mediated by the nuclear export signal.CRM1负责由核输出信号介导的细胞内运输。
Nature. 1997 Nov 20;390(6657):308-11. doi: 10.1038/36894.
6
The asymmetric distribution of the constituents of the Ran system is essential for transport into and out of the nucleus.Ran系统成分的不对称分布对于进出细胞核的运输至关重要。
EMBO J. 1997 Nov 3;16(21):6535-47. doi: 10.1093/emboj/16.21.6535.
7
Export of importin alpha from the nucleus is mediated by a specific nuclear transport factor.输入蛋白α从细胞核的输出由一种特定的核转运因子介导。
Cell. 1997 Sep 19;90(6):1061-71. doi: 10.1016/s0092-8674(00)80372-4.
8
CRM1 is an export receptor for leucine-rich nuclear export signals.CRM1是富含亮氨酸的核输出信号的输出受体。
Cell. 1997 Sep 19;90(6):1051-60. doi: 10.1016/s0092-8674(00)80371-2.
9
Exportin 1 (Crm1p) is an essential nuclear export factor.输出蛋白1（Crm1p）是一种重要的核输出因子。
Cell. 1997 Sep 19;90(6):1041-50. doi: 10.1016/s0092-8674(00)80370-0.
10
Evidence for a role of CRM1 in signal-mediated nuclear protein export.CRM1在信号介导的核蛋白输出中作用的证据。
Science. 1997 Oct 3;278(5335):141-4. doi: 10.1126/science.278.5335.141.
Zotero 插件