新兴的分子功能和 DEAD-box 蛋白 Dbp5/DDX19 在基因表达中的新作用。
Emerging molecular functions and novel roles for the DEAD-box protein Dbp5/DDX19 in gene expression.
机构信息
Biochemistry, Molecular, Cellular and Developmental Biology Graduate Group, University of California Davis, Davis, CA, USA.
Department of Viticulture and Enology, University of California Davis, Davis, CA, USA.
出版信息
Cell Mol Life Sci. 2021 Mar;78(5):2019-2030. doi: 10.1007/s00018-020-03680-y. Epub 2020 Nov 17.
Abstract
The DEAD-box protein (DBP) Dbp5, a member of the superfamily II (SFII) helicases, has multiple reported roles in gene expression. First identified as an essential regulator of mRNA export in Saccharomyces cerevisiae, the enzyme now has reported functions in non-coding RNA export, translation, transcription, and DNA metabolism. Localization of the protein to various cellular compartments (nucleoplasm, nuclear envelope, and cytoplasm) highlights the ability of Dbp5 to modulate different stages of the RNA lifecycle. While Dbp5 has been well studied for > 20 years, several critical questions remain regarding the mechanistic principles that govern Dbp5 localization, substrate selection, and functions in gene expression. This review aims to take a holistic view of the proposed functions of Dbp5 and evaluate models that accommodate current published data.
摘要
DEAD-box 蛋白 (DBP) Dbp5 是超级家族 II (SFII) 解旋酶的成员，在基因表达中具有多种报道的作用。该酶最初被鉴定为酿酒酵母中 mRNA 输出的必需调节剂，现在已报道具有非编码 RNA 输出、翻译、转录和 DNA 代谢的功能。该蛋白在各种细胞区室（核质、核膜和细胞质）中的定位突出了 Dbp5 调节 RNA 生命周期不同阶段的能力。尽管 Dbp5 已经被研究了 > 20 年，但关于控制 Dbp5 定位、底物选择和基因表达功能的机制原理仍有几个关键问题。本综述旨在全面评估 Dbp5 的拟议功能，并评估适应当前已发表数据的模型。
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