Madu Uju L, Ogundeji Adepemi O, Mochochoko Bonang M, Pohl Carolina H, Albertyn Jacobus, Swart Chantel W, Allwood J William, Southam Andrew D, Dunn Warwick B, May Robin C, Sebolai Olihile M
Pathogenic Yeast Research Group, Department of Microbial, Biochemical and Food Biotechnology, University of the Free State Bloemfontein, South Africa.
School of Biosciences, University of Birmingham Birmingham, UK.
Front Microbiol. 2015 Dec 9;6:1351. doi: 10.3389/fmicb.2015.01351. eCollection 2015.
We previously reported on a 3-hydroxy fatty acid that is secreted via cryptococcal capsular protuberances - possibly to promote pathogenesis and survival. Thus, we investigated the role of this molecule in mediating the fate of Cryptococcus (C.) neoformans and the related species C. gattii when predated upon by amoebae. We show that this molecule protects cells against the phagocytic effects of amoebae. C. neoformans UOFS Y-1378 (which produces 3-hydroxy fatty acids) was less sensitive toward amoebae compared to C. neoformans LMPE 046 and C. gattii R265 (both do not produce 3-hydroxy fatty acids) and addition of 3-hydroxy fatty acids to C. neoformans LMPE 046 and C. gattii R265 culture media, causes these strains to become more resistant to amoebal predation. Conversely, addition of aspirin (a 3-hydroxy fatty acid inhibitor) to C. neoformans UOFS Y-1378 culture media made cells more susceptible to amoebae. Our data suggest that this molecule is secreted at a high enough concentration to effect intracellular signaling within amoeba, which in turn, promotes fungal survival.
我们之前报道过一种3-羟基脂肪酸，它通过隐球菌荚膜突起分泌，可能是为了促进发病机制和生存。因此，我们研究了这种分子在变形虫捕食新型隐球菌和相关物种加氏隐球菌时介导其命运的作用。我们发现这种分子能保护细胞免受变形虫的吞噬作用。与新型隐球菌LMPE 046和加氏隐球菌R265（两者均不产生3-羟基脂肪酸）相比，新型隐球菌UOFS Y-1378（产生3-羟基脂肪酸）对变形虫的敏感性较低，并且向新型隐球菌LMPE 046和加氏隐球菌R265的培养基中添加3-羟基脂肪酸会使这些菌株对变形虫捕食更具抗性。相反，向新型隐球菌UOFS Y-1378的培养基中添加阿司匹林（一种3-羟基脂肪酸抑制剂）会使细胞更容易被变形虫捕食。我们的数据表明，这种分子以足够高的浓度分泌，以影响变形虫内的细胞内信号传导，进而促进真菌的生存。
