在一个过表达N-ras原癌基因的细胞系中，蛙皮素对肌醇1,4,5-三磷酸生成和细胞内钙释放的刺激作用增强。
Bombesin stimulation of inositol 1,4,5-trisphosphate generation and intracellular calcium release is amplified in a cell line overexpressing the N-ras proto-oncogene.
作者信息
Lloyd A C, Davies S A, Crossley I, Whitaker M, Houslay M D, Hall A, Marshall C J, Wakelam M J
机构信息
Institute of Cancer Research, Chester Beatty Laboratories, London, U.K.
出版信息
Biochem J. 1989 Jun 15;260(3):813-9. doi: 10.1042/bj2600813.
Abstract
Bombesin stimulation of T15 cells in which the inducible N-ras oncogene is overexpressed caused elevated production of inositol phosphates compared to uninduced cells [Wakelam, Davies, Houslay, McKay, Marshall & Hall (1986) Nature (London) 323, 173-176]. This elevated response is shown here to result from increased generation of inositol 1,4,5-trisphosphate leading to an elevated release of intracellular stored Ca2+. Single-cell analysis of Ca2+ release showed that the elevated response is not a consequence of an increased fraction of responding cells. These amplifications are consistent with p21N-ras acting like a guanine nucleotide coupling protein in this cell line.
摘要
与未诱导的细胞相比，用铃蟾肽刺激过表达可诱导型N-ras癌基因的T15细胞，会导致肌醇磷酸产量升高[瓦克莱姆、戴维斯、豪斯利、麦凯、马歇尔和霍尔（1986年），《自然》（伦敦）323, 173 - 176]。此处显示这种升高的反应是由于肌醇1,4,5 - 三磷酸生成增加，导致细胞内储存的Ca2+释放增加。对Ca2+释放的单细胞分析表明，升高的反应不是反应细胞比例增加的结果。这些放大作用与p21N-ras在该细胞系中像鸟嘌呤核苷酸偶联蛋白一样起作用是一致的。
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