Wakelam M J
Department of Biochemistry, University of Glasgow, Scotland.
FEBS Lett. 1988 Feb 8;228(1):182-6. doi: 10.1016/0014-5793(88)80613-6.
The bombesin-stimulated inositol phosphate response is only increased in cells transformed by the overexpression of N-ras when they are cultured under sub-confluent conditions. The inhibition of the amplified bombesin stimulation can be partially reversed by either, incubation of the cells for 5 h with 1 mM suramin, or by a 30 min preincubation of cells in excess buffer. The inhibitory effects are probably caused by the effects of autocrines and may explain some of the different published observations concerning the effects of ras gene products upon inositol phospholipid metabolism.
铃蟾肽刺激的肌醇磷酸反应仅在通过N-ras过表达转化的细胞中，于亚汇合条件下培养时增加。放大的铃蟾肽刺激的抑制可通过以下两种方法部分逆转：将细胞与1 mM苏拉明孵育5小时，或在过量缓冲液中对细胞进行30分钟预孵育。这些抑制作用可能是由自分泌的作用引起的，并且可能解释了一些关于ras基因产物对肌醇磷脂代谢影响的已发表的不同观察结果。
