Liu Liang, Gao Chao, Yao Ping, Gong Zhiyong
College of Food Science and Engineering, Wuhan Polytechnic University, Wuhan 430023, China ; Hubei Collaborative Innovation Center for Processing of Agricultural Products, Wuhan Polytechnic University, Wuhan 430023, China.
National Institute for Nutrition and Health, Chinese Center for Disease Control and Prevention, Beijing 100050, China.
Biomed Res Int. 2015;2015:607531. doi: 10.1155/2015/607531. Epub 2015 Dec 1.
A growing body of evidence has indicated that high-fat diet-induced nonalcoholic fatty liver disease is usually accompanied by oxidized low-density lipoprotein (ox-LDL) deposited in the liver. The current study aimed to investigate the effect of quercetin on high-fat diet-induced ox-LDL accumulation in the liver and to explore the potential underlying mechanisms. The results demonstrate that quercetin supplementation for 24 weeks significantly alleviated high-fat diet-induced liver damage and reduced hepatic cholesterol and ox-LDL level. Quercetin notably inhibited both mRNA and protein expression of CD36 (reduced by 53% and 71%, resp.) and MSR1 (reduced by 25% and 45%, resp.), which were upregulated by high-fat diet. The expression of LC3II was upregulated by 2.4 times whereas that of p62 and mTOR was downregulated by 57% and 63% by quercetin treatment. Therefore, the significantly improved autophagy lysosomal degradation capacity for ox-LDL may be implicated in the hepatoprotective effect of quercetin; scavenger receptors mediated ox-LDL uptake might also be involved.
越来越多的证据表明，高脂饮食诱导的非酒精性脂肪性肝病通常伴有肝脏中氧化型低密度脂蛋白（ox-LDL）的沉积。本研究旨在探讨槲皮素对高脂饮食诱导的肝脏中ox-LDL积累的影响，并探索其潜在的机制。结果表明，补充槲皮素24周可显著减轻高脂饮食诱导的肝损伤，并降低肝脏胆固醇和ox-LDL水平。槲皮素显著抑制了CD36（分别降低53%和71%）和MSR1（分别降低25%和45%）的mRNA和蛋白表达，高脂饮食使其上调。经槲皮素处理后，LC3II的表达上调了2.4倍，而p62和mTOR的表达分别下调了57%和63%。因此，槲皮素对ox-LDL自噬溶酶体降解能力的显著改善可能与其肝脏保护作用有关；清道夫受体介导的ox-LDL摄取可能也参与其中。
