Li Jian-shuang, Chen Zhi-yun, Jiang Jian-ping, He Bei-hui
Zhongguo Zhong Yao Za Zhi. 2015 Jul;40(13):2644-8.
This study aimed to investigate the involved immunologic mechanism of pure total flavonoids from Citrus (PTFC) on the development of non-alcoholic steatohepatitis (NASH). C57BL/6 mice were fed with high .fat diet for 16 weeks to induce the NASH model, and from the 7th week three dosages (25, 50 and 100 mg x kg(-1) x d(-1)) of PTFC were administrated intragastric for 10 weeks respectively. Serum TG, CHOL, ALT, AST were determined by biochemical assay, histopathological changes of the liver tissue were observed by HE staining, expression of RORyt and Foxp3 mRNA of the liver tissue was detected by Real-time PCR, and serum IL-17, IL-6, IL-10 and IL-4 were determined by.Cytometric Beads Array. As a result, we find that after the administration of PTFC, the in- flammation of the liver tissue of NASH mice was attenuated, liver function was improved, and the expression of RORgammat mRNA was higher in the liver tissue while which was lower of Foxp3 mRNA, the level of proinflammatory cytokines IL-17 and IL-6 decreased and the level of suppressive cytokines IL-10 and IL-4 increased. These data show that PTFC protects the development of NASH through regulating the Th17/Treg balance and attenuating inflammation.
本研究旨在探讨柑橘纯总黄酮（PTFC）对非酒精性脂肪性肝炎（NASH）发生发展的免疫机制。将C57BL/6小鼠喂饲高脂饮食16周以诱导NASH模型，从第7周起分别给予三种剂量（25、50和100mg·kg⁻¹·d⁻¹）的PTFC灌胃10周。通过生化检测法测定血清甘油三酯（TG）、胆固醇（CHOL）、谷丙转氨酶（ALT）、谷草转氨酶（AST），通过苏木精-伊红（HE）染色观察肝组织的组织病理学变化，通过实时荧光定量聚合酶链反应（Real-time PCR）检测肝组织中维甲酸相关孤儿受体γt（RORγt）和叉头框蛋白3（Foxp3）mRNA的表达，通过细胞计数微球阵列（Cytometric Beads Array）测定血清白细胞介素-17（IL-17）、白细胞介素-6（IL-6）、白细胞介素-10（IL-10）和白细胞介素-4（IL-4）。结果发现，给予PTFC后，NASH小鼠肝组织炎症减轻，肝功能改善，肝组织中RORγt mRNA表达升高而Foxp3 mRNA表达降低，促炎细胞因子IL-17和IL-6水平降低，抑炎细胞因子IL-10和IL-4水平升高。这些数据表明，PTFC通过调节Th17/Treg平衡和减轻炎症来保护NASH的发生发展。
