Silverman J A, Zurlo J, Watson M A, Yager J D
Department of Pharmacology and Toxicology, Dartmouth Medical School, Hanover, New Hampshire.
Mol Carcinog. 1989;2(2):63-7. doi: 10.1002/mc.2940020203.
The major objective of this study was to investigate the expression of members of the raf family of proto-oncogenes during rat liver regeneration. The steady-state level of expression of both c-raf-1 and A-raf-1 increased three- to fivefold 18-24 h following partial hepatectomy, and it returned to basal levels by 72 h. Expression of c-myc and Ha-ras mRNA was increased at 3 and 18-24 h, respectively, confirming previous reports. Increased steady-state levels of c-raf-1, A-raf-1, and Ha-ras mRNA were also detected in hepatocytes isolated from rat liver 24 h after partial hepatectomy. Thus, elevated expression of the raf genes closely correlated with that of Ha-ras, beginning at 12 h and reaching maximal levels during the first peak of DNA synthesis following partial hepatectomy.
本研究的主要目的是调查原癌基因raf家族成员在大鼠肝脏再生过程中的表达情况。部分肝切除术后18 - 24小时，c-raf-1和A-raf-1的稳态表达水平增加了三到五倍，并在72小时恢复到基础水平。c-myc和Ha-ras mRNA的表达分别在3小时和18 - 24小时增加，证实了先前的报道。在部分肝切除术后24小时从大鼠肝脏分离的肝细胞中也检测到c-raf-1、A-raf-1和Ha-ras mRNA的稳态水平升高。因此，raf基因表达的升高与Ha-ras的表达密切相关，从12小时开始，并在部分肝切除术后DNA合成的第一个高峰期间达到最高水平。
