Matsuzaki N, Okada T, Kameda T, Negoro T, Saji F, Tanizawa O
Department of Obstetrics and Gynecology, Osaka University Medical School, Japan.
Am J Reprod Immunol. 1989 Apr;19(4):121-7. doi: 10.1111/j.1600-0897.1989.tb00560.x.
An immunosuppressive factor released by choriocarcinoma cell lines was analyzed in the present study. It inhibited the proliferative responses of human T cells stimulated by lectins or alloantigens. It also blocked the generation of alloreactive cytotoxic T cells. The suppressive activity of the factor was detected in the responses of the T cells costimulated with 1 nM 12-O-tetradecanoyl phorbol 13-acetate and 1 microM A23187, suggesting the possibility that the factor acted on the intracellular signal transduction in T cells rather than interfering with early events such as T cell receptor signal transduction through cell membranes. Moreover, the factor acted directly on T cell proliferation pathways without activation of suppressor cells but did not act on T cell activation pathways. Taken together, all these findings expanded our previous reports on a factor released by normal trophoblasts, indicating the possible identity of the two factors. The physicochemical properties of the choriocarcinoma-derived factor were examined, and the biological significance of the factor during pregnancy was discussed in this paper.
本研究分析了绒毛膜癌细胞系释放的一种免疫抑制因子。它抑制凝集素或同种异体抗原刺激的人T细胞的增殖反应。它还阻断同种反应性细胞毒性T细胞的产生。在用1 nM 12 - O - 十四烷酰佛波醇13 - 乙酸酯和1 microM A23187共刺激的T细胞反应中检测到该因子的抑制活性，这表明该因子可能作用于T细胞内的信号转导，而不是干扰诸如通过细胞膜的T细胞受体信号转导等早期事件。此外，该因子直接作用于T细胞增殖途径，而无需激活抑制细胞，但不作用于T细胞激活途径。综上所述，所有这些发现扩展了我们之前关于正常滋养层细胞释放的一种因子的报道，表明这两种因子可能相同。本文研究了绒毛膜癌衍生因子的物理化学性质，并讨论了该因子在妊娠期间的生物学意义。
