月经子宫内膜和早孕蜕膜中微小RNA及靶基因的表达

miRNA and target gene expression in menstrual endometria and early pregnancy decidua.

作者信息

Lv Yang, Gao Shujun, Zhang Yuanyuan, Wang Liyan, Chen Xiujuan, Wang Yu

机构信息

The Key Laboratory of National Education Ministry for Mammalian Reproductive Biology and Biotechnology, Inner Mongolia University, Hohhot 010070, Inner Mongolia, China.

School of Life Sciences, Inner Mongolia University, Hohhot 010021, Inner Mongolia, China.

出版信息

Eur J Obstet Gynecol Reprod Biol. 2016 Feb;197:27-30. doi: 10.1016/j.ejogrb.2015.11.003. Epub 2015 Nov 27.

DOI:10.1016/j.ejogrb.2015.11.003

PMID:26699100

Abstract

OBJECTIVE

The role of miRNAs in modulating gene expression in decidualization remains to be determined. We performed a comparative study to identify miRNAs and their potential mRNA targets with different expression levels between endometrium and decidua.

METHODS

Quantitative reverse transcription-polymerase chain reaction (qRT-PCR) was used to measure the expression of the miR-146b-5p, miR-181b-5p, miR-424, miR-532, miR-199a-3p, miR-423, miR-22-3p, let-7i-5p, and miR-1 and the predicted target genes IGF2R, LEPR, SGK1, MMP2, MMP10, LIF, IL6, and STAT3 in menstrual endometria and early pregnancy decidua.

RESULTS

miR-146b-5p, miR-181b-5p, miR-424, miR-532, and miR-199a-3p were significantly downregulated in early pregnancy decidua, while miR-423, miR-22-3p, let-7i-5p, and miR-1 were significantly upregulated. In addition, the decidua had significantly lower levels of expression of LIF, IL6, MMP2, MMP10, and IGF2R and higher levels of expression of SGK1, LEPR, PROK1, and STAT3 than the menstrual endometria group.

CONCLUSION

Our results provide new insights into the expression of miRNAs that regulate genes involved in decidualization and the maintenance of early pregnancy.

摘要

目的

微小RNA（miRNA）在蜕膜化过程中调节基因表达的作用尚待确定。我们进行了一项比较研究，以鉴定在子宫内膜和蜕膜之间具有不同表达水平的miRNA及其潜在的mRNA靶标。

方法

采用定量逆转录-聚合酶链反应（qRT-PCR）检测月经子宫内膜和早孕蜕膜中miR-146b-5p、miR-181b-5p、miR-424、miR-532、miR-199a-3p、miR-423、miR-22-3p、let-7i-5p、miR-1以及预测的靶基因IGF2R、LEPR、SGK1、MMP2、MMP10、LIF、IL6和STAT3的表达。

结果

miR-146b-5p、miR-181b-5p、miR-424、miR-532和miR-199a-3p在早孕蜕膜中显著下调，而miR-423、miR-22-3p、let-7i-5p和miR-1显著上调。此外，与月经子宫内膜组相比，蜕膜中LIF、IL6、MMP2、MMP10和IGF2R的表达水平显著降低，而SGK1、LEPR、PROK1和STAT3的表达水平较高。